基于PCR的水稻候选RGA标记检验和评估  

Examination and evaluation of PCR-based candidate RGA markers in rice.

作　　者：肖天霞[1] 赵向前[1] 汪旭升[1] 吴为人[1] 朱军[1] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2007年第1期51-55,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30470916).

摘　　要：在生物信息学方法开发的基于e-PCR、共显性的402个水稻候选RGA标记的基础上,随机挑选40对引物,分别以水稻品种Nipponbare和93-11的DNA为模板进行PCR扩增验证.得到清晰条带占所用标记总数的87.5%(35个标记),其中31对引物为共显性标记,4对引物为显性标记.用这31对RGA引物在24个水稻品种中进行PCR扩增,结果表明标记具有很好的通用性和特异性.RGA引物平均标记多态性指标(PIC)值为0.46,标记具有较好的多态性.Based on 402 co-dominant rice candidate RGA markers developed by bioinformatics methods, 40 pairs of primers were randomly selected to test the polymorphisms in rice two subspecies, japonica and indica. The results showed that 87. 5% （35 markers） of the total number of markers obtained the clear bands, which 31 markers were co-dominant and 4 markers were dominant. In addition, 31 primer pairs were used to analyze 24 different japonica and indica varieties, and the result showed the markers have good commonality and subspecies-specificity, The averaged Polymorphism Information Content （PIC） of RGA markers was 0.46, indicated that the RGA markers have good polymorphism.

关 键 词：水稻 RGA标记 PCR 

分 类 号：Q7[生物学—分子生物学]