发根农杆菌rolC基因的克隆及序列分析  

Cloning of rolC Gene of Ri Plasmid and Sequence Analysis

作　　者：刘兴菊[1] 梁海永[1] 杨敏生[1] 周怀军[1] 

机构地区：[1]河北农业大学林学院,河北保定071000

出　　处：《西北林学院学报》2007年第2期72-75,共4页Journal of Northwest Forestry University

基　　金：河北省自然科学基金(302312)

摘　　要：以发根农杆菌30148为材料,设计了rolC基因的特异性引物,应用PCR技术,扩增出了正确序列的rolC基因585 bp片段,并利用pUCm-T载体对此片段进行了克隆,并进行了序列分析。所克隆rolC基因与已发表的序列相比较,核苷酸序列的同源性达到100%,但另一个克隆中部分碱基发生了改变,同源性达到99%。Ri plasmid was developed and a 585bp fragment of rolC gene was amplified by PCR via primer of rolC gene, and the fragment was cloned into vector pUCm-T. Comparison with previously published nucleotide sequence showed that the homology was 100%. But another clone gene nucleotide sequence showed that the homology was 99% with it.

关 键 词：RI质粒 ROLC基因 克隆 

分 类 号：S718.46[农业科学—林学]