RP-HPLC法同时测定沙地柏中3种鬼臼毒素类化合物的含量  

Quantitative Determination of the Podophyllotoxin Analogues in Sabina vulgaris by RP-HPLC

作　　者：陈艳[1] 李广泽[1] 曹雪峰[1] 陈存根[2] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心,陕西杨陵712100 [2]西北农林科技大学资源与环境学院,陕西杨陵712100

出　　处：《西北林学院学报》2007年第2期127-130,共4页Journal of Northwest Forestry University

基　　金：国家科技攻关计划"西部开发"重大项目(2004BA901A14);国家"863"计划项目(2001AA246016)

摘　　要：用RP—HPLC法同时测定了沙地柏3种鬼臼类化舍物（鬼臼毒素、脱氧鬼臼毒素和苦鬼臼毒素）的含量。采用YMC C13反相柱，甲醇：水（55：45）为流动相，紫外检测波长为290nm，鬼臼毒素和脱氧鬼臼毒素在62．50～1000．00ug／mL范围内的线性方程分别为：Y=（3．5024＋252．1221X）×10^-6，Y=（3．5805＋621．0468X）×10^-6；相关系数为r=0．9999和0．9992；回收率分别为100．932％（RSD=1．388％）和98．968％（RSD=1．108％）；苦鬼臼毒素在6．25-100．0ug／mL范围内的线性方程为：Y=（4．7818＋6369．7680X）×10^-6，相关系数r=0．9996，回收率为100．110％（RSD=1．344％）。沙地柏叶中的鬼臼毒素、脱氧鬼臼毒素和苦鬼臼毒素含量分别为3．894、3．345、0．427mg／g，茎中的含量分别为2．225、1．829、0．241mg／g；超临界流体萃取沙地柏叶中的3种化舍物含量分别为2．327、2．116、0．191mg／g，茎中的含量分别为1．784、1.446、0．108mg／g。该方法步骤简便，结果准确，分析速度快。A RP-HPLC method was developed for the simultaneous determination of podophyllotoxin, deoxypodophyllotoxin and picropodophyllotoxin in Sabina vulgaris. The chromatographic system consisted of ODS column and mobile phase of methanol-water（55 ： 45）; flow rate： 1 mL/min; detection wavelength ： 290 mn linear ranges of podophyllotoxin and deoxypodophyllotoxin were 62.50-1 000. 00ug/mL, the regression equation were Y= （3. 502 496＋252. 122 1X）×10^-6 and Y= （3. 580 590＋621. 046 8X） × 10^-6 respectively; r were 0. 999 9 and 0. 999 2; the recovery rate were 100. 932% （RSD=1. 388%） and 98. 986% （RSD=1. 108%） respectively. The linear range of picropodophyllotoxin was 6. 25-100. 00ug/mL, the regression equation was Y=（4. 781 883＋6369. 768 X） ×10^-6,r=0. 999 6, recovery rate was 100. 110% （RSD=1. 344%）. The contents of three compounds were 3. 894 mg/g,3. 345 mg/g and 0. 427 mg/g respectively in extracts with Soxhlet extraction from the leaves, and 2. 225 mg/g, 1. 829 mg/g and 0. 241 mg/g respectively from the stems. In extracts with SFE-CO2, the content of three compounds were 2. 327 mg/g, 2. 116 mg/g, 0. 191 mg/g respectively from the leaves, and 1. 784 mg/g, 1. 446 mg/g and 0. 108 mg/ g respectively from the stems. The method developed is simple, accurate, and rapid.

关 键 词：沙地柏 鬼臼毒素 脱氧鬼臼毒素 苦鬼臼毒素 RP—HPLC 

分 类 号：S791.440.8[农业科学—林木遗传育种]