雷公藤多苷对单侧输尿管结扎小鼠肾小管间质细胞表型转化的影响  

Effects of Tripterygium Wlilfordii on Transdifferentiation in Mice Kidney with the Unilateral Ureteral Obstruction

作　　者：杨汝春[1] 王永钧[1] 周大为[1] 林京莲[1] 王军[1] 朱晓玲[1] 

机构地区：[1]浙江中医药大学附属广兴医院杭州市中医院,浙江杭州310007

出　　处：《中华中医药学刊》2007年第2期290-292,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然基金资助项目(Y204488);浙江省中医药管理局资助项目(2003C124)

摘　　要：目的:通过观察雷公藤多苷对UUO小鼠肾小管间质细胞表型转化的影响,探讨抗风湿中药在防治肾间质纤维化中的作用。方法:采用小鼠单侧输尿管结扎(UUO)模型,用雷公藤多苷进行干预,用血管紧张素转化酶抑制剂蒙诺作为对照。肾组织中α-平滑肌肌动蛋白(α-smoothmuscleactin,α-SMA)、波形蛋白(Vimentin)蛋白表达采用免疫组化或Western-blotting方法检测;基因表达采用逆转录-聚合酶链式反应(RT-PCR)方法。结果:UUO模型组小鼠肾间质纤维化程度以及α-SMA、Vimentin蛋白表达和基因表达水平均较假手术组显著增高,而雷公藤和蒙诺治疗均可显著降低肾间质细胞表型转化。结论:抗风湿药雷公藤多苷可显著降低肌成纤维细胞的积聚,可能是防治肾间质纤维化的分子机制之一。Objectlve： To investigate the effects of Tripterygium Wlilfordii on transdifferentiation of renal interstitial cells in mice kedney with the Unilateral Ureteral Obstruction（UUO） by examining the expression of α - smooth muscle actin（at-SMA）, Vimentin. Methods： Mice were randomly assigned to shame operation group （shame proup ）, UUO group, Tfipterygium Wlilfordii and ACEI treatment group after UUO. The protein expression of α- SMA, Vimentin were assessed by immunohistochemistry and Western - blotting;at - SMA,Vimentin mRNA were detected by using reverse transcription- polymerase chain reaction analyses（ RT -PCR）. And the change of renal pathology was observed at the same time. Results： In comparison with the shame group, the protein levels of at - SMA, Vimentin of UUO mice and treatment groups increased significantly, meanwhile Tripterygium Wlilfordii and ACEI could significantly reduce the expression of at - SMA, Vimentin on levels of protein. The effects of Tripterygium Wlilfordii on Vimentin were more significant than ACEI. Conclusion： Tripterygium Wlilfordii could reduce the transdifferentiation of renal interstitial cells and prevent renal interstitial fibrosis.

关 键 词：雷公藤多苷 表型转化 Α-平滑肌肌动蛋白 波形蛋白 

分 类 号：R33[医药卫生—人体生理学]