山核桃提取物体外抗肿瘤作用研究  

The Anti-tumor Effects of Carya Cathayensis Sarg. Extracts in Vitro

作　　者：胡旭姣[1] 赵肖君[1] 周奋[1] 余美荣[1] 陈银银[1] 李瑞枝[1] 贾永慧[1] 丁志山[1] 

机构地区：[1]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2007年第2期369-370,共2页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:探讨山核桃的抗肿瘤作用。方法:采用极性溶剂法从山核桃叶子中分离提取出石油醚、氯仿、乙酸乙酯、正丁醇和水提取物5种提取物,采用MTT法观察其对肿瘤细胞SGC-7901增殖的抑制作用,并采用光学显微镜和电镜方法观察乙酸乙酯提取物对SGC-7901细胞凋亡的诱导作用。结果:山核桃乙酸乙酯提取物抗肿瘤作用最强,并且能够诱导肿瘤细胞凋亡。结论:山核桃叶子中有抗肿瘤物质,值得进一步深入研究。Objective： To study the anti-tumor effects of Carya Cathayensis Sarg. Methods ： We extract five kinds extracts from Carya Cathayensis Sarg. leaves with light petroleum, chloroform,acetic ether, n-butanol and water. We observe the anti-tumor effects of them by MTT assay. And we also observe the induction of apoptosis of acetic ether extracts on SGC- 7901. Results： The acetic ether extracts have the strongest anti-tumor effects, and it can induce SGC-7901 cell apeptosis. Conclusion： There is antitumorigcnic substance in Carya Cathayensis Sarg. leaves, and it worth of study further more.

关 键 词：山核桃 抗肿瘤 乙酸乙酯提取物 凋亡 

分 类 号：R931[医药卫生—生药学]