干细胞移植对慢性心功能不全兔心室肌细胞膜Na^+-K^+泵和肌浆网Ca^(2+)泵活性的影响  

Changes of Sarcolemma Na^+/K^+ ATPase and Sarcoplasmic Reticulum Membrane Ca^(2+) ATPase Activity after Stem Cell Transplantation in Chronic Heart Failure

作　　者：范忠才[1] 陈茂[1] 邓珏琳[1] 刘小菁[2] 张立[1] 饶莉[1] 杨庆[1] 黄德嘉[1] 

机构地区：[1]四川大学华西医院心内科 [2]四川大学华西医院心血管疾病研究室,成都610041

出　　处：《生物医学工程学杂志》2007年第1期136-139,181,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30270566)

摘　　要：研究经冠状动脉灌注移植自体骨髓单个核细胞(BMCs)、间充质干细胞(MSCs)和骨骼肌成肌细胞(SMs)对CHF兔CMNKA和SERCA的影响,探讨干细胞移植治疗CHF的确切机制。使用阿霉素制作兔CHF模型,经双球囊封堵主动脉根部后分别灌注BMCs、MSCs、SMs和无血清DMEM液。4周后处死动物,比较干细胞移植后CMNKA和SERCA活性变化。与对照组相比,假手术组CMNKA和SERCA活性降低(P<0.05)。不同类型的干细胞移植后心室肌CMNKA活性均明显改善(P分别小于0.05、0.01和0.01)。干细胞移植各组SERCA有改善的趋势,但与假手术组相比,差异无统计学意义(P均大于0.05)。说明干细胞移植能够改善衰竭心室肌细胞膜Na+-K+ATP酶和肌浆网Ca2+ATP酶的异常,这可能是其治疗心力衰竭的重要机制之一。To assess the changes of sarcolemma Na^＋/K^＋ ATPase （CMNKA） and sarcoplasmic reticulum membrane Ca^2＋ ATPase （SERCA） activities after stem cells transplantation in heart failure. Rabbit was used as heart failure model by intravenously injecting adriamycin. Autologous bone marrow mononuclear cells （BMCs）, bone marrow mesenchymal stem cells （MSCs） or skeletal myohlasts （SMs） were introduced into coronary arteies through the root of aorta when two balloons occluding just above sinus of Valsalva. After 4 weeks, left ventricular ejection fraction （LVEF）was evaluated by echocardiography, and the activities of CMNKA and SERCA were measured by colorimeter. In BMCs （n = 8）and MSCs （n=8） group, LVEF were significantly improved （P〈 0. 05）. No significant improvement were seen in SMs group （n=6） compared to sham group（n=8）. The CMNKA activity in all stem cells groups was significantly increased compared to sham group （P〈0. 05）. Meanwhile, in comparison with sham group, the incremental tendencies of SERCA activity were seen in stem cells groups. In conclusion, stem cells transplantation could increase the activities of CMNKA and SERCA in heart failure, a possible mechanism to improve heart function.

关 键 词：心功能不全 干细胞移植Na^+-K^+ATP酶 Ca^2+ATP酶 

分 类 号：R541.6[医药卫生—心血管疾病]