植物材料冷冻蚀刻技术的几点改善  

Some Improvment of Freeze-etch Technique on Plant Tissue

作　　者：郭一松[1] 陈一新[1] 吴敦肃 

机构地区：[1]中国科学院上海植物生理研究所

出　　处：《电子显微学报》1990年第4期41-45,共5页Journal of Chinese Electron Microscopy Society

摘　　要：植物材料的冷冻蚀刻制样过程中遇到的技术难处是很多的。我们采用减压蒸发冷却法制备的液氮泥作样品的高速冷却剂、用5～10M高浓度铬酸作试样的腐蚀剂和在用聚四氟乙烯材料制成的凹型器皿中将样品进行腐蚀和漂洗复型膜。用以上改善的方法制备的样品没有冰晶,腐蚀彻底的复型膜不卷曲、制样成功率极高。There are some technical difficulties during preparing plant tissue for freeze--etch technique. In the pr^--- sent paper, we make use of nitrogen slush made by means of reducing the presure, evaporating andcondens-ing, to cryofixate the sample very quickly, use 5-10M concentrated CrO3 as a corrosive, and clean the replica in a concave-shape utensils made of polytetrafluoroethylene. Using the improved method, we get a better re-sult: The sample has no ice crystal, and the replica does not fold over. Therefore, the successful chance is raised greatly.

关 键 词：植物材料 冷冻蚀刻 样品 制备 

分 类 号：Q－34[生物学]