Using the SELDI ProteinChip System to Detect Changes in Protein Expression in Vero Cells after Infection  

作　　者：Zhi-jun LIU Bin WANG Zhi-yong YAN Xu-xia SONG Dong-meng QIAN Zhi-qiang BAI 

机构地区：[1]Department of Microbiology, Qingdao Medical College, and Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao 266021,China

出　　处：《中国病毒学》2007年第1期68-73,共6页Virologica Sinica

基　　金：National Natural Science Foundation of China (30540075);Mt. Tai Scholar Construction Engineering Special Foundation of Shandong province

摘　　要：人的疱疹单一的病毒(HSV-1 ) 1 引起美容，眼睛，并且 encephalitic 疾病并且与潜伏的感染和癌症被联系。这里，我们开发了由使用 SELDI 蛋白质薄片在在 vitro 有教养的 Vero 房间检测蛋白质表示的变化在蛋白质水平学习 HSV-1 感染的致病的一个工具。在有为 12， 24 或 48 h 的 HSV-1 和文化的感染以后，房间被收获并且 lysed。IMAC3 数组被用于 SELDI-TOF-MS 在感染前后检测 proteomic 差别。薄片检测了一系列差别表示蛋白质山峰。有趣地，在 16 912 Da 的山峰和 17 581 Da 与 ISG15 的分子的团精确相应，它可以在感染的过程期间参予抗病毒的活动。因此，我们获得了的结果能用作一个基础学习在病毒和它的主人之间的 HSV-1 和相互作用的致病。另外，他们能为 HSV-1 感染的治疗在新治疗学的目标的发现帮助。关键词 SELDI 蛋白质薄片 - Vero 房间 - HSV-1 - 蛋白质表达式 CLC 数字 R373 基础项目：中国(30540075 ) 的国家自然科学基础；Human herpes simplex virus 1 （HSV-1） causes facial, ocular, and encephalitic disease and is associated with latent infection and cancer. Here, we developed a means of studying the pathogenesis of HSV-1 infection at the protein level by using the SELDI Protein Chip to detect changes of protein expression in Vero cells cultured in vitro. After infection with HSV-1 and culture for 12, 24 or 48 h, cells were harvested and lysed. IMAC3 arrays were applied to SELDI-TOF-MS to detect proteomic differences before and after infection, The chip detected a series of differentially expressed protein peaks. Interestingly, both peaks at 16 912 Da and 17 581 Da corresponded precisely with the molecular mass of ISG15, which may participate in antiviral activity during the process of infection. Thus, the results we obtained can serve as a basis to study the pathogenesis of HSV-1 and the interaction between the virus and its host. In addition, they can help in the discovery of new therapeutic targets for treatment of HSV-I in- fection.

关 键 词：SELDI Protein Chip Vero Cells HSV-1 Protein Expression 

分 类 号：Q[生物学]