Affymetrix基因芯片技术分析尖锐湿疣患者皮损局部免疫状态的研究  

Analysis of Local Immunity in CA Lesions with DNA Microarray Technique

作　　者：王飞[1] 相文中[2] 毕志刚[2] 李光富[3] 刘丰[3] 张兆松[3] 

机构地区：[1]东南大学附属中大医院皮肤科,江苏南京210009 [2]南京医科大学第一附属医院皮肤科,江苏南京210029 [3]南京医科大学分子生物研究所,江苏南京210029

出　　处：《中国皮肤性病学杂志》2007年第1期8-9,44,共3页The Chinese Journal of Dermatovenereology

基　　金：东南大学博士启动基金(编号:2006-134);东南大学中大医院科研基金(编号:2005YJ04)

摘　　要：目的探讨尖锐湿疣(CA)患者局部免疫状态,分析局部Th1/Th2的变化。方法采用Affymetrix基因芯片技术分析CA皮损局部多个细胞因子和抗体变化,并用Real-timeQ-PCR验证部分结果。结果CA皮损局部Th1细胞因子IFN-γ,IL-12,TNF-α较正常包皮明显降低,Th2细胞因子IL-4,IL-10较正常包皮升高,IL-6降低,IL-2,IL-5,IL-13无明显变化;sIgA,IgA,IgG,IgM较正常包皮高。结论CA患者局部Th1/Th2细胞失衡,Th细胞向Th2极化,CA局部处于Th2优势应答状态。Objective To study the local immune and analysis the Th1/Th2 response in condyloma acuminatum（CA） lesion. Methods To analysis the levels of cytokine and antibody in CA local lesions with DNA microarray technique,and verify the results with Real-time Q-PCR. Results Compared with the normal control , the intensities of Thl （ IFN-γ, IL-12, TNF-α） and Th2 （ IL-4, IL-10） type cytokines were lower and higher, respectively, and no change was found of IL-2, IL-5, IL-13, The transcript level of sIgA,IgA,IgG,IgM was increased, The data of Real-time Q-PCR was consistent with the microarrarys. Conclusion The imbalance of Th1/Th2 response in CA lesions was verified,representing immune suppression of Th1 response and over activation of Th2 response.

关 键 词：尖锐湿疣 基因芯片 人乳头瘤病毒 免疫 

分 类 号：R752.5[医药卫生—皮肤病学与性病学]