溶胶-凝胶法制作塑料基DNA提取芯片以及应用于从人血中提取DNA  

Plastic Based DNA Extraction Chip Fabricated with Sol-Gel Method and Its Application to Extract DNA from Human Blood

作　　者：刘惯超[1] 肖宏[1] 李荔[2] 郭旻[2] 邢婉丽[1] 程京[1] 

机构地区：[1]清华大学生物科学与技术系 [2]北京博奥生物芯片有限责任公司,北京102206

出　　处：《材料科学与工程学报》2007年第1期122-124,98,共4页Journal of Materials Science and Engineering

基　　金：国家863计划功能基因组和生物芯片重大专项资助项目(2002AA2Z2011)

摘　　要：本文用溶胶-凝胶法在塑料表面制作二氧化硅涂层,并利用制作的二氧化硅层来提取DNA。利用这种方法制作了塑料基的DNA提取芯片,并将该芯片应用于从人血中提取DNA。结果表明:经过溶胶涂渍、在内表面制作二氧化硅层的芯片能够实现从血液中提取DNA,而没有经过溶胶涂渍的芯片无法提取DNA。涂渍的芯片所提取出的DNA能够满足聚合酶链反应的需要。In this paper, silicon oxidation layer was fabricated on plastic surface using sol-gel method, and the fabricated SiO2 layer was used to extract DNA. Using this method, plastic based DNA extraction chips were fabricated, and the chips were applied to extract DNA from human blood. Experimental results indicate that, the chip which was coated using the sol could extract DNA from the blood, while the chip which was not coated could not extract DNA. It was validated that the extracted DNA could meet the need of polymerase chain reaction （PCR）.

关 键 词：溶胶-凝胶法 二氧化硅 DNA提取芯片 

分 类 号：R318.08[医药卫生—生物医学工程]