大剂量甲基泼尼松龙对大鼠脊髓损伤早期单核细胞趋化蛋白-1表达水平和巨噬细胞浸润的影响  

Effects of large dose Methylprednisolone on level of MCP-1 expression and macrophages infiltration in early spinal cord injured rats

作　　者：马胜忠[1] 汤继文[1] 张雪松[1] 张晶涛[1] 

机构地区：[1]山东大学齐鲁医院骨伤科,济南250012

出　　处：《临床神经病学杂志》2007年第1期45-47,共3页Journal of Clinical Neurology

摘　　要：目的探讨大剂量甲基泼尼松龙(MP)对脊髓损伤早期单核细胞趋化蛋白-1(MCP-1)表达水平和巨噬细胞浸润的影响。方法采用Nystrm法制备大鼠脊髓损伤模型。将72只雄性SD大鼠随机分为脊髓损伤组、生理盐水对照组、MP治疗组,每组又分为3个亚组,分别于损伤后1d、3d、7d取材。进行HE染色及形态学观察;免疫组化检测MCP-1阳性细胞、巨噬细胞的分布。并对以上3组各个时间点的细胞数进行计数及比较。结果MP治疗组的MCP-1阳性细胞、巨噬细胞数明显少于其他两组,而Olsiwski小体的平均密度值增加(均P<0.05)。结论脊髓损伤早期MP可以减少损伤区域MCP-1的表达水平及巨噬细胞的浸润,并减轻脊髓继发性损伤的程度。Objective To explore the effects of Methylprednisolone （MP） on level of monocyte chemoattractant protein 1 （MCP-1） expression and macrophages infiltration in early spinal cord injured （SCI） rats. Methods Animal SCI model was established by modified Nystroem method. 72 rats were randomly divided into three groups： SCI group, normal saline control group and MP treatment group. Each group, divided into three subgroups, were sacrificed at 1 d, 3 d, 7 d postinjury. Spinal cord tissues were collected and sections were made to do following examinations： （1）morphological observation by hematoxylin and eosin stain;（ 2 ）the expression and distribution of local MCP-1 positive ceils, and the infiltration of macrophages by immunohistochemical method. The cell population of different time points were counted and compared. Results MP reduced the proportion of both MCP-1 positive ceils and marerophages in local spinal cord, meanwhile MP elevated the mean density of Olsiwski corpuscle at all time points as compared with those in control group （all P 〈 0. 05 ）. Conclusions MP can provide neurotrophic function by inhibiting both the level of MCP-1 expression and the macrophages infiltration, thereby meliorating the degree of secondary injury after SCI.

关 键 词：脊髓损伤 甲基泼尼松龙 单核细胞趋化蛋白-1 巨噬细胞 

分 类 号：R651.2[医药卫生—外科学]