小鼠脑垂体前叶细胞线形溶酶体的高压电镜观察  

ULTRACYTOCHEMICAL OBSERVATION OF NEMATOLYSOSOMES IN RAT ANTERIOR PITUITARY CELLS

作　　者：于向民[1] 刘友章[2] 

机构地区：[1]青岛医学院组织学与胚胎学教研室 [2]广州中医学院内科学教研室

出　　处：《青岛医学院学报》1996年第4期297-298,共2页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：①目的探讨脑垂体前叶细胞线形溶酶体的功能及与圆形溶酶体之间的关系。②方法应用高压电镜细胞化学方法,观察小鼠脑垂体前叶5种内分泌细胞中酸性磷酸酶阳性的线形溶酶体。③结果线形溶酶体存在于脑垂体前叶5种内分泌细胞中,线形结构可一侧膨大或与圆形溶酶体相连并发出分支互相连接,形成一个立体的网状结构。④结论线形溶酶体并不是一种特殊类型的溶酶体,线性结构及其相连的圆形溶酶体可以认为是一个整体。Objective To study the function of nematolysosomes and the relationship between the thread-like structures and the spherical lysosomes in anterior pituitary cells of mouse. Method Ultracytochemical high voltage electron microscopy (HVEM) was used to observe the ACPase positive nematolysosomes in five different types of secretory cells in anterior pituitary. Result Nematolysosomes were present in five different types of secretory cells. The thresd-like structure connected with spherical lysosome and gave branches which linked up with each other to form three dimensional network in the cytoplasm. Conclusion The nematolysosomes are not a specialized population of lysosome, but appear to be a functional intermediate. The thread-like structure and the spherical lysosome belong to the same structural integrity.

关 键 词：脑垂体前叶细胞 线形溶酶体 酸性磷酸酶 垂体 

分 类 号：Q244[生物学—细胞生物学]