Bax基因在不同组织细胞中的表达  

Differential Expression of Bax Gene in the Various Human Tissues and Cell Lines

作　　者：陈亚兵 曾桂生 蔡毓 俞春东 陈华 温龙平 

出　　处：《厦门大学学报（自然科学版）》1996年第6期998-1000,共3页Journal of Xiamen University：Natural Science

摘　　要：Ｂａｘ基因在不同组织细胞中的表达陈亚兵，曾桂生，蔡毓，俞春东，陈华，温龙平（肿瘤细胞工程国家专业实验室）近年来，细胞编程死亡已成为细胞生物学研究的热点．细胞编程死亡是在组织、器官、系统发育成熟过程中发生的一种自杀性细胞死亡现象，无功能或老化细胞通过此...Here is reported the differential expression pattern of Bax gene in the various tissues and cell lines. Total RNA isolated from human brain, liver, testis, thymus, spleen and placenta, as well as total RNA isolated from four human cell lines (neuroblastoma Sk cell line,CNE cell line, MGc-803 cell line and HL-60 cell line )were reverse-transcribed into cDNA and used for PCR amplification with a part of oligonucleotide primers specific for Bax coding region.A 0. 6 kb fragment, expected to code for Bax-a, was observed in tissue samples, indicting a wide expression pattern and a fundamental role for Bax-a. None of the tissues expressed any detectable Bax-βor bax-γ mRNA. Bax expression in the cell lines was more diverse. In MGc-803 and SK cells, only the 0. 6 kg Bax-a was detected, even though the expression in SK cells was low. On the other hand, HL-60 cells expressed multiple forms of Bax mRNA, resulting in multiple PCR amplification bands: a 0. 6 kb bax-a,a 1. 1 kb Bax-β, two Bax-γ (1. 15 kb and 0. 55 kb)、and three unknown bands (0. 2 kb, 0. 4 kb and 0. 5 kb). No PCR bands were observed in the CNE sample.

关 键 词：BAX基因 基因表达 细胞编程死亡 细胞生物学 

分 类 号：Q28[生物学—细胞生物学]