cDNA文库构建及其在植物抗性研究中的应用  

Construction of cDNA Library and its Application in the Study on Plant Resistance

作　　者：许兰珍[1] 何永睿[1] 姜国金[1] 刘小丰[1] 陈善春[1] 

机构地区：[1]中国农业科学院柑桔研究所

出　　处：《安徽农业科学》2007年第3期660-662,664,共4页Journal of Anhui Agricultural Sciences

基　　金：科技部"973"重大基础研究前期研究专项基金(2005CCA02900);重庆市自然科学基金(SCTC2005BA1010)

摘　　要：cDNA文库是指生物不同生长发育时期特定组织或器官所转录的全部mRNA经反转录形成的cDNA与载体连接后形成的克隆的集合,cDNA文库是在基因组水平上研究某一生物特定器官、组织和发育时期基因表达的前提和基础。笔者就近年来cDNA文库的构建及其在植物抗性研究中的应用作一综述。cDNA library is the pool of cDNA fragments which are ligated with vectors, and cDNA fragments are reverse- transferred from total mRNAs of some organism at certain period, cDNA library has the important significance in the research on, the differential gene expressing of different tissues and growth-periods, In the paper the kind of cDNA library construction was simply summarized and the application of cDNA library in the study on plant resistance was described.

关 键 词：CDNA文库 植物抗性 基因克隆 基因表达 EST 

分 类 号：Q343.1[生物学—遗传学]