Long interspersed elements in three species of Gracilaria (Gracilariaceae, Rhodophyta)  

作　　者：李玉晖 姚建亭 高天翔 段德麟 

机构地区：[1]Department of Aquaculture, Ocean University of China, Qingdao 266003, China Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China [2]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China [3]Department of Aquaculture, Ocean University of China, Qingdao 266003, China [4]Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

出　　处：《Chinese Journal of Oceanology and Limnology》2007年第1期59-66,共8页中国海洋湖沼学报（英文版）

基　　金：Supported by the NSFC (No. 40376049 and No. 30500383);Shandong Agricultural Seed Stock Breeding Project and Knowledge In-novation Program of Chinese Academy of Sciences (KSCX2-YW-N-47-02).

摘　　要：In order to find out whether long interspersed elements （LINEs） existed in macro-algae gehomes or not, we tested the LINE homologues in representative families （species）： Gracilaria （G. eucheumoides Harv., G. tenuistipitata Chang et Xia, and G. textorii （Sur） De-Toni）, Laminaria （L. longissima Miyabe and L. japonica Aresch.）, and Ulva （U. lactuca L. and U. pertusa Kjellm.） during 2004 to 2005. Polymerase Chain Reaction （PCR） was carried out with degenerate oligonucleotide primers designed from LINEs of rice homologues and Cin4 of maize. Cloning and nucleotide sequencing of the PCR products revealed that 4 clones that derived from 3 species of Gracilaria have LINE homologues. The nucleotide sequences of the 4 LINE homologues diverged greatly, but the amino acid sequences deduced from them were relatively conserved. The endonuclease regions of the LINE homologues greatly diverged from that of other plants, but they had closer phylogenetic relationship to Zepp elements in Chlorella sp., which indicated that sequence divergence by vertical transmission has been a major influence on the evolution of algal LINEs.In order to find out whether long interspersed elements (LINEs) existed in macro-algae ge- nomes or not, we tested the LINE homologues in representative families (species): Gracilaria (G. eucheumoides Harv., G. tenuistipitata Chang et Xia, and G. textorii (Sur) De-Toni), Laminaria (L. longis- sima Miyabe and L. japonica Aresch.), and Ulva (U. lactuca L. and U. pertusa Kjellm.) during 2004 to 2005. Polymerase Chain Reaction (PCR) was carried out with degenerate oligonucleotide primers de- signed from LINEs of rice homologues and Cin4 of maize. Cloning and nucleotide sequencing of the PCR products revealed that 4 clones that derived from 3 species of Gracilaria have LINE homologues. The nucleotide sequences of the 4 LINE homologues diverged greatly, but the amino acid sequences deduced from them were relatively conserved. The endonuclease regions of the LINE homologues greatly di- verged from that of other plants, but they had closer phylogenetic relationship to Zepp elements in Chlor- ella sp., which indicated that sequence divergence by vertical transmission has been a major influence on the evolution of algal LINEs.

关 键 词：Endonuclease gene long interspersed element （LINE） PHYLOGENY GRACILARIA GRACILARIACEAE RHODOPHYTA 

分 类 号：Q946[生物学—植物学]