不同浓度葡萄糖对MG63细胞株护骨素和护骨素配体及其相关因子表达的影响  

Effects of different concentrations of glucose on the expressions of osteoprotegerin,the ligand of osteoprotegerin and related cytokines in MG63 cells

作　　者：周玮 姬秋和[2] 张南雁[2] 王加林 

机构地区：[1]解放军第八二医院内分泌科,江苏淮安223001 [2]第四军医大学西京医院内分泌科,陕西西安710032

出　　处：《医学研究生学报》2007年第2期146-149,共4页Journal of Medical Postgraduates

摘　　要：目的:观察不同葡萄糖浓度环境下人成骨肉瘤MG63细胞株护骨素(OPG)、护骨素配体(OPGL)及其相关因子如肿瘤坏死因子相关凋亡诱导配体(TRAIL)、巨噬细胞集落刺激因子(M-CSF)、转化生长因子β(TGF-β)的表达。方法:用RT-PCR法检测基因表达情况。结果:高糖能下调MG63细胞中OPG及TGF-β的表达,上调OPGL、M-CSF和TRAIL的表达。结论:高糖环境可能导致成骨细胞中OPG及TGF-β的表达减少,OPGL、M-CSF和TRAIL等细胞因子的表达增多,使破骨细胞的数目和活性增加,骨吸收增强和骨量丢失,这可能是糖尿病骨质疏松症的一个重要的发病机制。Objective ：To observe the regulative effects of different concentrations of glucose on the expressions of osteoprotegerin （ OPG）, the ligand of osteoprotegerin （OPGL） and the related cytokines [ tumor necrosis factor related apoptosis inducing ligand （TRAIL）, macrophage colony-stimulating factor（M-CSF） and transforming growth factor β（TGF-β） ] in osteosarcoma MG63 cells. Methods：The expressions of OPG,OPGL, M-CSF,TRAIL and TGF-β mRNA was examined by reverse transcriptase （RT）-PCR. Results ： High concentration glucose up-regulated the expression of OPGL, M-CSF and TRAIL but downregulated OPG and TGF-β expression in the MG63 cells. Conclusion：One of the key pathogenetic factors of diabetic osteoporosis is that high concentration glucose leads to the down-regulated expression of OPG and TGF-β but the up-regulated expression of some bone-resorbing cytokines such as OPGL, M-CSF and TRAIL in osteoblasts, then stimulates osteoclast differentiation and activity, which potentiates bone resorption and bone loss.

关 键 词：高糖 MG63细胞 护骨素 

分 类 号：R587.2[医药卫生—内分泌]