低功率超声对家兔卵巢微血管的影响  

Effect of low power ultrasound on ovarian angiogenesis of rabbit

作　　者：高迎春[1] 蒋小青[2] 吴巍[3] 

机构地区：[1]东南大学临床医学院妇产科,江苏南京210009 [2]江苏省中医院生殖中心,江苏南京210036 [3]东南大学应用医学研究所,江苏南京210009

出　　处：《医学研究生学报》2007年第2期153-154,159,I0005,共4页Journal of Medical Postgraduates

基　　金：国家"十五"科技攻关计划基金资助项目(批准号:2004BA706B03-01)

摘　　要：目的:探讨低功率超声辐射微泡剂对家兔卵巢微血管的影响,为该方法在临床上的进一步应用提供理论依据。方法:40只生育期的家兔随机分为四个实验组和一个对照组,每组8只家兔。由家兔一侧耳缘静脉注射微泡剂,然后以低功率低频率(0.5W)超声照射家兔腹部的4个象限,按照射时间长短分为30s组,60s组,120s组和240s组。照射结束24h后解剖四个实验组和对照组各4只家兔,取双侧卵巢,10%甲醛固定后进行苏木精-伊红(H-E)染色,观察家兔卵巢微血管内血栓形成情况。各组另4只家兔继续饲养,30d后解剖取双侧卵巢。结果:超声干预后24 h解剖的四个实验组家兔,双侧卵巢的微血管内偶可见血栓,其血栓形成率分别为(3.67±0.83)%、(3.54±1.07)%、(3.39±1.09)%和(3.92±1.10)%,各组间比较无显著性统计学差异(P>0.05)。而超声干预后饲养30d解剖的4个实验组家兔卵巢微血管内均未见明显的损伤。各组卵巢内的细胞饱满,无细胞核碎裂、固缩等细胞死亡征象。结论:本实验结果可初步认为低功率超声辐射微泡剂对家兔卵巢正常微血管似无明显影响。Objective ： To explore the effect of low power ultrasound on ovarian angiogenesis of rabbits. Methods：Forty rabbits received CO2 micro-bubble agent injection through one ear peripheral vein, after which their abdomen were radiated with low power ultrasound （0.5 W）, according to radiation time, ani- mals were divided into 4 experimental groups and 1 control group. A group received radiation for 30 seconds, B group was 60 seconds, C group was 120 seconds and D group was 4 minutes, control group was not radiated. After the radiation, 4 rabbits of every group were anatomized, their double ovaries were fixed with formalin, ovarian angiogenesis embolization was observed with HE. Other rabbits were breeded for one month before ovarian angiogenesis were observed in the same methods. Results ： After the radiation, four rabbits of every group were anatomizedin 24 hours, thrombus could be seen in the ovaries of the four groups, but they were few. The thrombus formation ratio of every group were （3.67 ± 0.83） %, （3.54 ± 1.07 ） %, （3.39 ± 1.09） % and （3.92 ± 1. 10 ） % respectively. Compared with the controlled group, the result from the experimental group was not significant （P 〉0.05）; After one month, thrombus could not be seen in the ovaries of all four groups. No injury could be seen in all kinds of cells in the four groups. Conclusion ： Rabbit ovarian angiogenesis was not affected by low power ultrasound.

关 键 词：低功率超声 微泡剂 微血管 血栓 

分 类 号：R445.1[医药卫生—影像医学与核医学]