核因子NF-κB对气道平滑肌细胞增殖的影响  

Effects of nuclear factor kappa-B(NF-κB) on proliferation of airway smooth muscle cells

作　　者：宋颖芳[1] 张耀亭[1] 李焕章[2] 

机构地区：[1]解放军第175医院南京军区结核病中心,福建漳州363000 [2]第四军医大学西京医院呼吸内科

出　　处：《西北国防医学杂志》2007年第1期32-34,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:研究核因子-κB(NF-κB)对大鼠气道平滑肌细胞(ASMC)增殖活性的影响。方法:体外培养ASMCs,采用EMSA法直接检测ASMC中NF-κB的活性,MTT法、细胞周期检测及细胞核增殖抗原(PCNA)的免疫细胞化学染色法观察细胞增殖情况。结果:血小板活化因子刺激后ASMC中NF-κB明显活化,各细胞增殖指标均显著增高(P<0.05),NAC可显著抑制上述效应(P<0.05)。结论:NF-κB的活化能直接参与ASMC的增殖。Objective：To investigate the effect of nuclear factor - kappa B （NF -κB ） on the proliferation of airway smooth muscle cells （ASMC）. Methods： NF -κB activity in ASMC was examined by electrophoretic mobility shift assay （EMSA）. MTF, flow cytometry and immunohistochemistry for proliferating cell nuclear antigen （PCNA） were used to evaluate ASMC proliferation. Results： Platelet - activating factor （PAF） stimulated the NF -κB activity and the cell proliferation in ASMC. The positive rate of PCNA was significantly different among these groups. N - acety - L - cysteine （ NAC ） markedly inhibited the increased NF -κB activity and ASMC proliferation induced by PAF. Conclusion： NF -κB activation may directly involve in the proliferation of ASMC, suggesting its important role in airway remodeling.

关 键 词：气道平滑肌细胞 增殖 核因子-ΚB N-乙酰半胱氨酸 血小板活化因子 

分 类 号：R563.9[医药卫生—呼吸系统]