胰蛋白酶酶切EPO之多肽片段抑制EPO促CFU-E形成的研究  

Effects of hydrolysates of EPO on stimulating colony-forming unit-erythropoiesis

作　　者：黄学文[1] 李素芝[2] 牟信兵[2] 高钰琪[1] 陈有[2] 龙仁玲[2] 黄文超[2] 李旬[2] 孙泽平[2] 洪勇[2] 石泉贵[2] 郭俊[2] 郑健保[2] 潘丽[2] 何贵强[2] 董学文[2] 

机构地区：[1]第三军医大学高原军事医学系,重庆400038 [2]西藏军区总医院,西藏拉萨850003

出　　处：《西南国防医药》2007年第1期12-15,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：拉萨市科技局资助合作项目(2004078)

摘　　要：目的:观察胰蛋白酶酶切EPO之多肽片段能否抑制EPO促红系形成。方法:对大鼠骨髓进行红系定向体外培养,加入胰蛋白酶酶切EPO之多肽片段干预,观察红系集落形成单位(CFU-E)之数量,判定胰蛋白酶酶切EPO之多肽片段对红系增殖活性的影响。结果:胰蛋白酶酶切EPO之多肽片段加入干预培养后,CFU-E数量明显减少(P<0.01)。结论:胰蛋白酶酶切EPO之多肽产物体外能对抗EPO,抑制红系增殖。其可作为治疗高原红细胞增多症的可能药物进一步研究。Objectlve：To observe the effects of the hydrolysates of erythropoietin（EPO） on stimulating colony - forming unit - erythropoiesisin in cultured marrows from rats. Methods： Marrows were collected from rats and the mononuclear cells in the marrows were isolated for erythroid oriented culture. Then the level of CFU - E formation was examined under the conditions treated with EPO or EPO＋ hydrolysates of EPO. Results： It was found that the CFU - E formation was significantly suppressed by the hydrolysates of EPO, Conclusion：The hydrolysates of are able to inhibit the activity of EPO stimulating colony - forming unit - erythroid proliferation in vitro.

关 键 词：促红细胞生成素 红系集落形成单位 骨髓 

分 类 号：R555.1[医药卫生—血液循环系统疾病] Q556.9[医药卫生—内科学]