环糊精-十二烷基硫酸钠胶束电动色谱分离光学异构体的模型及色谱条件优化  

Separation Model of Enantiomers Using Micellar Electrokinetic Chromatography With Cyclodextrin-Sodium Dodecyl Sulphate and Optimization of the Operation Conditions

作　　者：刘玉[1] 顾俊岭[1] 傅若农[1] 

机构地区：[1]北京理工大学化工与材料学院,北京100081

出　　处：《分析化学》1996年第12期1365-1370,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金;国家教委博士点基金

摘　　要：建立了环糊精-十二烷基硫酸钠胶束电动色谱中可电高及电中性-光学异构体的分离模型。通过计算机模拟预测了多种操作因素对手性分离可能产生的影响，并通过对丹磺酰化氨基酸及十八甲基炔诺酮旋光异构体的分离对理论预测进行了验证。研究表明，环糊精及SDS浓度对旋光异构体的分离有很大影响。利用该理论模型可以指导选择和优化实验条件。A separation model of ionizable and neutral enantiomers with cyclodextrin-sodium dodecyl sulphate (SDS) micellar electrokinetic chromatography was established. The effects of various operating conditions on chiral separation were predicted through computer simulation. The theoretical prediction was tested and verified using the separation of dansylated amino acid and norgestrel enantiomers. The experiments indicated that the concentrations of cyclodextrin and SDS affect the separation of enantiomers strongly. Using the theoreitical model can predicate and optimize operating condition of isomer separation.

关 键 词：胶束电动色谱 光学异构体 分离模型 环糊精 

分 类 号：O652.6[理学—分析化学]