单纯疱疹病毒抗原的电化学酶免疫法检测及其分型  

Electrochemical Enzyme Immunoassay for the Detection and Type Identification of Herpes Simplex Virus Antigen

作　　者：郑莉[1] 胡荫华[2] 宋俊峰[2] 

机构地区：[1]西安石油学院化工系,西安710061 [2]西北大学电分析化学研究所,西安710069

出　　处：《分析化学》1996年第10期1142-1146,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金

摘　　要：将酶联免疫吸附试验（ELISA）与单扫描示波极谱法（LSP）检测相联接，用自制的小型“DME－Pt－Ag／AgCl”三电极系统，与商品酶联板配套，直接检测酶促反应电活性产物，建立了ELISA－LSP法检测单纯疱疹病毒（HerpesSimplexVirus，HSV）抗原并进行分型的方法。用该法检测HSV－2抗原，检测限比ELISA试验低2～4倍．对40份临床标本进行检测，其结果与病毒分离法的阳性一致率（100％）高于ELISA与病毒分离法的阳性一致率（82．4％）。对共同检出的阳性标本进行分型，ELISA-LSP法与ELISA的结果完全相符。A double sandwich enzyme linked immunosorbent assay utilizing linear sweep polarographic detection (ELISA-LSP) for the detection and type identification of herpes simplex virus (HSV) antigen was established. Horseradish peroxidase(HRP) as a labelling enzyme catalyzes the convertion of o-phenylenediamine to 2, 2' -diaminoazobenzene (DAA)- At pH 10a well defined cathodic peak of DAA at Ep-800 mV(vs. Ag/AgCl) was achieved. Under optimum conditions, the titer of HSV-2 antigen determined by ELISA-LSP was 2 to 4 times lower than that by ELISA. Forty clinical specimens were detected and identified by ELISA-LSP and ELISA respectively. The tally ratio of ELISA-LSP to virus isolation was 100%, which was higher than that of ELISA(82. 4% ). The results of type identification to positive specimens by ELISA-LSP were in agreement with those by ELISA.

关 键 词：单纯疱疹病毒 极谱法 酶联免疫吸附 抗原 电化学 

分 类 号：R373.11[医药卫生—病原生物学] R392.11[医药卫生—基础医学]