2,6-二氯靛酚钠动力学分光光度法测定西红柿及草莓中的抗坏血酸  

Kinetic Spectrophotometry for the Determination of L-ascorbic Acid in Tomato and Strawberry by 2, 6-Dichlorophenol Indophenol Sodium

作　　者：秦永惠[1] 逯凯霄[1] 魏继华 李爱信 

机构地区：[1]河北大学化学系,保定071002

出　　处：《分析化学》1996年第10期1156-1158,共3页Chinese Journal of Analytical Chemistry

摘　　要：本文提出用2，6－二氯靛酚纳（以下简写DIS）作氧化剂动力学分光光度测定L－抗坏血酸的新方法。DIS与L－抗坏血酸混合．在固定时间间隔于611nm处测得吸光度A值的变化（△A／△t）与抗坏血酸含量成线性关系。线性范围为0～12mg／L。在样品处理时采用偏磷酸作为提取剂，以减缓抗坏血酸被氧化的速度结果表明，该方法有较高灵敏度，且操作简便，试剂易得，容易推广，是测定水果和蔬菜中L-抗坏血酸的好方法。This article gives a new method for the determination of L-ascorbic acid in tomato and strawberry by kinetic spectrophotometry. 2, 6-Dichlorophenol indophenol sodium (DIS)is acted as an oxidizing agent and metaphosphoric acid is used as an extractant to decrease the oxidation rate of ascorbic acid. The L-ascorbic acid and DIS are mixed and the absorbance at 611 nm is recorded at fixed intervals. The △A /△t is linear with the ascorbic acid percentage.The determination range is from 0 to 12 mg/L. The method has high sensitivity. simple manipulation and the reagent is easily available. The results indicate that this method is a good method for the determination of L-ascorbic acid in fruit and vegetable.

关 键 词：二氯靛酚钠 分光光度法 西红柿 草莓 维生素C 

分 类 号：O629.4[理学—有机化学] TS207.3[理学—化学]