无环鸟苷和鸟嘌呤的高效毛细管电泳测定  

Determination of Acyclic Guanosine and Guanine by High Performance Capillary Electrophoresis

作　　者：张书胜[1] 陈勇[1] 袁倬斌[1] 

机构地区：[1]中国科学技术大学研究生院,北京100039

出　　处：《分析化学》1996年第10期1212-1215,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金;中国科学院长春应用化学研究所电分析化学开放研究实验室基金

摘　　要：对无环鸟苷和鸟嘌呤的高效毛细管电泳（HPCE）分离测定条件进行了详细研究，建立了以2－氨基－5－巯基－3，4－噻二唑为内标的快速、灵敏的HPCE定量分析方法。当无环鸟苷和鸟嘌呤的浓度分别在10～300mg／L和5～300mg／L范围时，可进行定量分析，最低检出限分别为8．52mg／L和2．80mg／L。该方法用于无环鸟苷产品的质量检测，结果准确。用于尿液中的无环鸟苷和鸟嘌呤的快速测定，相对标准偏差小于4．5％。无环鸟苷和鸟嘌呤的回收率分别优于81％和86％。In this paper, the separation and determination conditions of acyclic guanosine and guanine by high performance capillary electrophoresis have been studied systematically. A simple and reproducible HPCE method for determining acyclic guanosine and guanine in acyclic guanosine product and urine is presented. 2-amino-5-mercapto-3, 4-dithiazole was used as an internal standard. Results showed that the linear ranges of determination for acyclic guanosine and guanine are 10~300 mg/L and 5-300 mg/L, and the detection limits are 8. 52mg/L and 2. 80 mg/L (S/N= 2) respectively. The recoveries are more than 81% for acyclic guanosine and 86% for guanine in urine. RSD is less than 4. 5%.

关 键 词：高效毛细管电泳 无环鸟苷 鸟嘌呤 尿 

分 类 号：R978.7[医药卫生—药品]