一种新的四糖柴胡皂苷结构的NMR研究  

The Structure Study of a New Saikosaponin

作　　者：马立斌[1] 金怡珠 涂光忠[1] 罗何生[2] 赵玉英[2] 胡长风[2] 张如意[2] 来鲁华[3] 徐筱杰[3] 唐有祺[3] 

机构地区：[1]北京微量化学研究所,北京100091 [2]北京医科大学药学院植化室,北京100083 [3]北京大学化学系,北京100871

出　　处：《化学学报》1996年第12期1200-1208,共9页Acta Chimica Sinica

摘　　要：本文报道从小叶黑柴胡(Bupleurum Smithii Wolff Var.Parvitolium Shan et Y.Li)分离出的一种新皂苷,应用COSY,CH-COSY,TOCSY,HMQC-COSY,HMBC等NMR方法对其结构进行了研究,确定该化合物为3β,16β,23,28-四羟基齐墩果烷-11,13(18)-二烯-3-O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃葡萄糖基-(1→6)-[β-D-吡喃葡萄糖基-(1→2)]-β-D-吡喃葡萄糖苷(1).命名为柴胡皂苷O(Saikosapnin O).Saikosaponin O was isolated from Bupleutum smithii Wolff var. parvifolium Shan et Y. Li. Its structure was determinated by NMR methods. From TOCSY and DQF - COSY spectra, the residues of monosaccharides and all their assignments were acquired. The anomeric configurations were obtained by 3JH1 H2 . The HMQC - COSY spectrum wasused successfully for13 C assignment since it was difficult to do it only by CH - COSY spectrum. All the glucosidic linkages were obtained from HMBC spectrum based on the 1H and 13C assignments NOESY spectrum also gave the concordant result about the configurations and the linkage positions.

关 键 词：柴胡皂苷 TOCSY HMQC-COSY HMBC NMR 

分 类 号：O629.13[理学—有机化学]