44种生物碱类化合物对人胃癌细胞株BGC和人肝癌细胞株BEL-7402细胞增殖抑制活性的筛选  

Inhibitory Effects of 44 Alkaloids Compounds Against Growth of Human Gastric Carcinoma Cell Line BGC and Human Hepatic Carcinoma Cell Line BEL-7402 in Vitro

作　　者：杨秀伟[1] 冉福香[1] 王瑞卿[1] 吴军[1] 彭俊[1] 裴惠平[1] 孙士勇[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100083

出　　处：《中国现代中药》2007年第2期6-9,共4页Modern Chinese Medicine

基　　金：科技部新医药博士基金资助项目

摘　　要：目的:从中药中筛选有抗肿瘤活性的化合物。方法:应用人胃癌细胞株BGC和人肝癌细胞株BEL-7402筛选44种生物碱类化合物对其增殖的抑制作用。结果:伊波加因、哈尔明碱、黄连碱、小檗碱、延胡索碱、小檗胺、吐根碱、马兜铃酸I、番茄碱苷、澳洲茄次碱、茄次碱、金鸡宁、罂粟碱和酒石酸麦角胺对人胃癌细胞株BGC细胞的增殖有一定程度的抑制作用,且呈浓度-效应关系;最强者为黄连碱、延胡索碱、小檗胺、吐根碱、番茄碱苷、澳洲茄次碱和金鸡宁,其次为伊波加因、哈尔明碱、小檗碱、马兜铃酸I、茄次碱、罂粟碱和酒石酸麦角胺。蛇根精、γ-崖椒碱、黄连碱、小檗碱、延胡索碱、吐根碱、马兜铃酸I、茄次碱和喜树碱对人肝癌细胞株BEL-7402细胞的增殖有一定程度的抑制作用,且呈浓度-效应关系;最强者为吐根碱、小檗碱、延胡索碱和喜树碱,其次为黄连碱、马兜铃酸I和茄次碱。结论:上述生物碱类化合物可能是含有该生物碱、有抗肿瘤活性中药的有效成分,亦可能成为开发抗肿瘤新药的候选药物。Objective： To screen antitumor active compounds from traditional Chinese materials medica. Methods： Forty -four alkaloids compounds isolated from the traditional Chinese materials medica were studied for their antitumor activity in vitro by determining the inhibitory percentage against growth of human gastric carcinoma cell line BGC and human hepatic carcinoma cell line BEL- 7402, respectively. Results： Ⅰt showed that ibogaine, e, berbamine, emetine, aristolochic acid Ⅰ, tomatine, solasodine, solani- dine, cinchonine, papaverine and （ - ） - ergotamine - L - tartrate inhib line BGC cell and their activity showed dose - effect relationship, which berbamine, emetine, tomatine, solasodine and cinchonine were stronger ited growth of human gastric carcinoma cell inhibitory effects of coptisine, corydaline, more than ibogaine, harmine, berberine, aristolochic acid Ⅰ, solanidine, papaverine and （ - ） - ergotamine - L - tartrate. And sarpagine, ~ - fagarine, coptisine, berberine, corydaline, emetine, aristolochic acid Ⅰ, solanidine and camptothecin inhibited growth of human hepatic carcinoma cell line BEL -7402 cell and their activity showed dose - effect relationship, which inhibitory effects of emetine, berberine, corydaline and camptothecin were stronger more than coptisine, aristolochic acid Ⅰ and solanidine. Conclusion： The alkaloids compounds above are likely to be effective constituents in corresponded traditional Chinese materials medica to inhibit growth of tumor cells or regarded potent candidating or leading compounds for research and development of anti - tumor drugs.

关 键 词：中药 生物碱类 人胃癌细胞株BGC 人肝癌细胞株BEL-7402 

分 类 号：R289.5[医药卫生—方剂学]