严重急性呼吸综合征患者病变组织中凋亡相关基因Fas/FasL的表达及其意义  

Expression of Fas/FasL and their significance in the pathological tissues of SARS patients

作　　者：贺莉[1] 张庆玲[1] 丁彦青[1] 

机构地区：[1]南方医科大学南方医院病理科,广东广州510515

出　　处：《中国实用内科杂志》2007年第4期276-277,共2页Chinese Journal of Practical Internal Medicine

基　　金：国家自然科学基金资助项目(30340015);中德SARS合作基金(GZ228-202-1)

摘　　要：目的观察并探讨凋亡相关基因Fas/FasL在严重急性呼吸综合征(SARS)病变组织肺、脾、淋巴结中的表达及其在SARS发病中的作用。方法2003年3月至5月,应用免疫组织化学方法对南方医科大学南方医院病理科4例SARS患者尸检组织及正常对照组织(取自非SARS患者尸检个体)肺、脾、淋巴结进行检测。结果与正常组织相比,SARS肺组织肺泡上皮细胞、支气管上皮细胞、巨噬细胞;脾脏及淋巴结淋巴细胞及单核巨噬细胞Fas,FasL表达增强。结论Fas/FasL过度表达在SARS病变组织中普遍存在,提示Fas/FasL介导的凋亡途径在SARS发病过程中可能起一定作用。Objective To study the mechanisms and expressions of Fas/FasL in the lungs, spleens and lymph nodes of SARS patients. Methods The lungs,spleens and lymph nodes specimens of four autopsy cases and one control subject were studied with immunohistochemical mehod. Results Compared with normal tissues,apoptotic cells increased significantly in the spleens, lungs and lymph nodes of SARS patients. The apoptotic cells of lungs included alveolar epithelial ones, bronchial epithelial ones, monocytes/macrophages and lymphocytes. Apoptosis in the spleens and the lymph nodes was observed primarily in monocytes/macrophages and lymphocytes, Conclusion Over-expression of Fas/FasL is a general phenomenon in SARS,suggesting that Fas/FasL may play a key role in the pathogenesis of SARS.

关 键 词：严重急性呼吸综合征 免疫组织化学 FAS/FASL 细胞凋亡 

分 类 号：R5[医药卫生—内科学]