番茄侧根原基发生相关基因RSI-1在烟草中功能的初步研究  

Function of the Lateral Root Development Relating Gene RSI-1 from Tomato in Tobacco Morphogenesis

作　　者：王友华[1,3] 卢孟柱[2] 李召虎[1] 王敏杰[2] 田晓莉[1] 翟志席[1] 段留生[1] 

机构地区：[1]中国农业大学农业与生物技术学院,植物生理与生物化学国家重点实验室 [2]中国林业科学院林业研究所 [3]南京农业大学农学院,南京210095

出　　处：《农业生物技术学报》2006年第6期905-910,I0018,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30200169);高校博士点基金项目(No.20010019008)资助。

摘　　要：为探讨番茄(Lycopersiconesculentumvar.cerasiforme)侧根原基发生相关基因RSI-1在形态发育过程中的作用及其在获得具超量侧根的转基因作物的应用前景,研究分析了RSI-1基因在烟草(Nicotianatabacum)中超量、反义抑制和RNA干扰表达后植株形态及转基因植株内侧根发生相关植物激素含量的变化特征。结果显示,RSI-1超量表达后,植株株高下降,叶片数和分支数有明显增加,而RSI-1反义抑制和RNA干扰表达后植株形态及赤霉素、生长素、脱落酸和玉米素及核苷的含量均没有发生显著变化。推测RSI-1的表达可以促进植物分枝的发生,但其在高等植物中的同源性可能较低,利用该基因定向改造弱根系作物时还需结合根特异启动子的应用。To investigate the function of the lateral root primordium development relating gene RSI-1 and the application possibility in super-root crops breeding, plasmids pBI-SRSI and pBI-ARSI that would lead to constitutive transcription of sense RSI-1 and anti-sense RSI-1 in plant cells and the RSI-1 RNAi interfere vector pIOOg-RSI-RNAi were constructed and transferred to tobacco （Nicotiana tabaccum） respectively. The transformants with the target chiermic gene constitutively transcribed were screened out by antibiotics medium selecting and Sourthem blotting. Then the morphological variations of the aboveground tissues were analyzed,and the change of the phytohormones involving in rooting was also studied. It was shown that divarication and lateral root formation was enhanced in the transgenic tobacco plants with RSI-1 over-expressed, while the morphology and the contents of gibberellic acid, indole-3-acetic acid, abscisic acid, zeatin and its ribosides were not altered significantly in the tobaccos with the RSI-1 antisense inhibited and RNA interfered. Based on the working mechanism of the function gene over-expressing, antisense inhibiting and RNA interfering, the evidences further conformed that RSI-1 is a gene involved in divaricating and it may be lowly conservative in evaluation. Thus, the root-specific promoters are needed when RSI-1 is used to modify root-deficient crops.

关 键 词：RSI-1 侧根原基 烟草 激素 功能 

分 类 号：S188[农业科学—农业基础科学]