利用SSR标记分析柑橘雄性不育及低育资源的遗传多样性  

Genetic Diversity of Male Sterile and Low Fertile Germplasm of Citrus Reealed Using SSR Markers

作　　者：曹庆芹[1] 孟海军[1] 文晓鹏[1] 伊华林[1] 邓秀新[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《农业生物技术学报》2006年第6期937-941,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.30070079)资助。

摘　　要：采用SSR标记分析了43份柑橘(Citrus)不育、低育、含不育胞质及13份可育材料的遗传多样性。结果表明,8对SSR引物扩增得到35个等位基因,平均每个位点有4.4个等位基因。利用NTSYSpc2.10统计分析软件,UPGMA法聚类分析表明,56份柑橘资源可分为3组。宽皮柑橘类品种为第一组,甜橙、葡萄柚、椪柑及杂柑类品种为第二组,包含不育胞质的澳洲指橘为第三组。SSR聚类分析还表明本地广橘与温州蜜柑有着紧密的亲缘关系,而与早橘、槾橘、本地早等的亲缘关系较远?涕?不育与低育资源的遗传多样性分析将为此类资源的收集保存及应用提供依据。Genetic diversity of 43 accessions of male sterile, low fertile, containing sterile cytoplasm and 13 fertile ones in Citrus were assessed using simple sequence repeat markers （SSRs）. Thirty- five polymorphic alleles, with an average of 4.4 per primer, were generated from eight primers. Cluster analysis was performed via un-weighted pair group method analysis （UPGMA） method using the NTSYS-pc version 2.10. The results showed that three groups could be obtained from those accessions. The cultivars of mandarin were classified into group 1; those of sweet orange, grapefruit, ponkan or tangor into group 2; Microcitrus with male sterile cytoplasm into group 3. Cluster analysis also revealed that satsuma mandarin had closer relatedness with Bendiguangju mandarin than with Zaoju, Manju, Bendizao tangerine. Genetic diversity of male sterile and low fertile Citrus will provide useful information for further collection, preservation and utilization.

关 键 词：柑橘 雄性不育 SSR标记 遗传多样性 

分 类 号：S188[农业科学—农业基础科学] S184