荔枝体细胞胚性培养物的DNA提取方法和RAPD分析  

Genomic DNA Extraltion of Cultures and RAPD Analysis in the Process of Somatic Embryogenesis in Litchi

作　　者：黄素华[1] 赖钟雄[2] 

机构地区：[1]福建农林大学亚热带果树研究所,福州350002 [2]福建省龙岩学院生物科学与工程系,福建龙岩364000

出　　处：《热带作物学报》2006年第4期56-59,共4页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(39870544);霍英东教育基金会高等学校青年教师基金(71026)等资助项目的部分内容

摘　　要：采用补加PVP的SDS法,提取得到高质量的荔枝(Litchi chinensis Sonn)胚性培养物基因组DNA。对荔枝胚性培养物的RAPD分析结果表明:荔枝胚性和非胚性愈伤组织、玻璃化胚和白色胚、不同染色体数目的细胞系类型之间在DNA分子标记上存在差异,证实荔枝胚性愈伤组织继代及再分化过程中存在遗传物质的变异。High-quality genomic DNA was extracted from litchi embryogenic cultures by the SDS method plus PVP. The RAPD analysis showed that there were differences in DNA molecular markers among the embryogenic and nonembryogenic calli, the vitrified and the white embryoids and the three screened cell lines with different chromosome numbers, which proved that there existed variation in hereditary substances during the process of subcultures and differentiation of embryogenic calli in litchi.

关 键 词：荔枝 胚性培养物 体胚发生 DNA提取 RAPD 遗传变异 

分 类 号：S667.101[农业科学—果树学] Q813.11[农业科学—园艺学]