早期肠内谷氨酰胺补给对烫伤大鼠回肠p-ERK通路的影响  

Effect of Initial enteral glutamine supplementation on p-ERK1/2 pathway in ileal mucosa of scalded rats

作　　者：郭光华[1] 邓志云[1] 范骏[1] 张红艳[1] 李国辉[1] 

机构地区：[1]南昌大学第一附属医院烧伤中心,江西省南昌市330006

出　　处：《世界华人消化杂志》2007年第4期353-358,共6页World Chinese Journal of Digestology

基　　金：江西省自然科学基金项目;No.0340078

摘　　要：目的：观察早期肠内谷氨酰胺补给对烫伤大鼠回肠p-ERK1／2表达的影响．方法：选健康SD大鼠88只随机分为肠内营养组（EN组，n=40）、肠内营养加谷氨酰胺补给组（EN＋C1n组，n=40）和伤前对照组（n=8），制备总烧伤面积（TBSA）为30％的Ⅲ^0烫伤动物模型，伤后早期分别给予肠内营养制剂能全力和能全力加G1n，于6，12，24，48和96h观察肠黏膜细胞P-ERK的表达分布及血浆D-乳酸浓度变化．结果：烧伤后血浆D-乳酸水平升高,EN组在观察时间内血浆D-乳酸水平没有恢复正常（P〈0．05），EN＋G1n组血浆D-乳酸水平在伤后48h恢复至伤前水平且明显低于EN组（4．7±0．9mmol／Lvs6．9±1．2mmol／L．P〈0．05）．p-ERK1／2免疫组化染色显示烧伤后阳性细胞数量显著增加，EN＋G1n组回肠阳性细胞百分比在24，48，96h明显高于EN组（绒毛：87．6％±9．6％，84．4％±10．3％，74．6％±9．7％vs64．6％±7．3％，59．6％±7．1％，58．4％±7．4％．P〈0．05；隐窝：73．6％±11．2％，67．4％±8．6％．63．6％±7．9％vs54．3％±6．3％，51．6％±5．9％．48．4％±5．3％，P〈0．05）．Western blot显示EN组伤后6h p-ERK1／2表达明显增加，12h达高峰，然后逐渐下降，EN＋G1n在24h达到高峰并逐渐下降．结论：相对于肠内营养，早期肠内给予G1n能促进肠上皮细胞p-ERK1／2的表达，改善黏膜局部的屏障功能．AIM： To investigate the effect of initial enteral glutamine supplementation on p-ERK1/2 expression in ileal mucosa of scalded rats. METHODS： Healthy adult Sprague Dawley rats were randomly divided into enteral nutrition group （n = 40）, enteral nutrition plus glufamine （EN ＋ Gin） group （n = 40） and pseudoscalded （PS） group （n = 8）. Scalded model （30% total body surface area, Ⅲ^0 scald injury） was established in the rats of the former two groups, and the corresponding methods were used. The expression of p-ERK1/2 in ileal mucosa was analyzed by immunohistochemical staining and Western blot, and the changes of plasma D-lactate concentration in portal vein were measured by ultraviolet spectrophotometry. RESULTS： The concentration of D-lactate was heightened after scalding, and it restored to the normal level after 48 hours in EN ＋ Gin group, which was significantly lower than that in EN group （4.7 ±0.9 mmol/L vs 6.9 ±1.2 mmol/L, P 〈 0.05）. Immunohistochemistry revealed the number of p-ERK1/2-positive cells had been increased after scalded injury, and the percentages of p-ERK1/2-positive cells had been markedly higher in EN ＋ Gin group than that in EN group at the 24^th, 48^th, and 96^th hour （villus： 87.6% ± 9.6%, 84.4% ± 10.3%, 74.6% ± 9.7% vs 64.6% ± 7.3%, 59.6% ± 7.1%, 58.4% ± 7.4%, P 〈 0.05; recess： 73.6% ± 11.2%, 67.4% ± 8.6%, 63.6% ± 7.9% vs 54.3% ± 6.3%, 51.6% ± 5.9%, 48.4% ± 5.3%, P 〈 0.05）. Western blot showed that the expression of p-ERK1/2 had been obviously increased in EN group at the 12^th hour after scalded injury, and then decreased gradually, while it reached the peak at 24^th hour, and then decreased gradually in EN ＋ Gin group. CONCLUSION： In comparison with enteral nutrition, enteral glutamine supplementation can promote the expression of p-ERK1/2 in ileal mucosa and improve the function of intestinal barrier in scalded rats.

关 键 词：肠内营养 谷氨酰胺 P-ERK1/2 D-乳酸 

分 类 号：R644[医药卫生—外科学]