向日葵二萜化学成分及其细胞毒活性研究(英文)  

Diterpenes from Helianthus annuus and their cytotoxicity in vitro

作　　者：索茂荣[1] 田泽[1] 杨峻山[1] 吕扬[2] 吴立[2] 

机构地区：[1]中国医学科学院,中国协和医科大学药用植物研究所,北京100094 [2]中国医学科学院,中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2007年第2期166-170,共5页Acta Pharmaceutica Sinica

基　　金：National Natural Science Foundation of China(20432030)

摘　　要：为了研究向日葵(Helianthus annuusL.)的生物活性成分。本文采用色谱法系统研究向日葵的化学成分,根据波谱学分析鉴定了化合物的结构,用MTT法评价其细胞毒活性。从成熟的向日葵花盘中分离鉴定了11个化合物,分别为:对映贝壳杉-2α,16α-二醇(1),对映贝壳杉-15α,16α-环氧-17-醛-19-酸(2),对映贝壳杉-16β-醇(3),phyllocladan-16β-ol(4),ent-atisan-16α-ol(5),15-羟基-对映贝壳杉-16-烯-19酸(6),对映贝壳-15-当归酰氧基-16-烯-19-酸(7),对映贝壳杉-16-烯-19酸(8),对映贝壳杉-17-羟基-15-烯-19酸(9),对映贝壳杉-16β,17-二羟基-19酸(10)和ciliaric acid(11)。其中化合物1和2为新化合物,部分化合物显示一定的细胞毒活性。To search for bioactive compounds from the flower disc of Helianthus annuus L., chromatography was used to isolate and purify the chemical constituents, their structures were identified by spectral analysis, MTT method was applied to investigate their cytotoxic activities, some compounds showed moderate cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines. Eleven compounds were obtained from the flower disc of H. annuus, and identified as ent-kaurane-2α, 16α-diol （1） and entkaurane-15α, 16α-epoxy-17-al-19-oic acid （2） , and nine known diterpenes, ent-kaurane-16β-ol （3） , phyllocladan-16β-ol （4） , ent-atisan-16α-ol （5） , grandifloric acid （6） , angeloylgrandifloric acid （7） , ent-kaurane-16-en-19-oic acid （8）, ent-kaurane-17-hydroxy-15-en-19-oic acid （9）, ent-kaurane-16β,17- dihydroxy-19-oic acid （10）, and ciliaric acid （11）. Compounds 1 and 2 are new compounds, some compounds showed cytotoxic activities on SF-268, MCF-7 and HepG2 cell lines.

关 键 词：向日葵 二萜 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]