体外扩增兔内皮细胞种植异体血管移植的实验研究  

Experimental Study of Vascular Allograft of Autologous Venous Endothelial Cells in Seeded Rabbits

作　　者：何茂鑫[1] 赵渝[1] 

机构地区：[1]重庆医科大学附属第一医院血管外科,重庆400016

出　　处：《中国普外基础与临床杂志》2007年第1期72-75,共4页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨自体静脉内皮细胞衬里异体血管移植的可行性。方法将体外扩增培养的兔静脉内皮细胞铺植于经处理的异体血管,体外培养9d,然后行异体静脉原位移植术。结果经传代培养收获的内皮细胞总数为(9.47±0.35)×106个。细胞种植3h后异体血管腔面可见有内皮细胞附着,但未形成完整内膜;9d时腔面被一层连续的单层内皮细胞覆盖,细胞形态饱满,平行排列,呈梭形。术后8周所有移植段血管保持畅通。结论预衬自体内皮细胞的异体血管可保持长期的通畅性。Objective To study the feasibility of transplanting autologous venous endothelial cells, as the liner, to the allogenic vein and to investigate the patency rate after such transplantation. Methods Autologous endothelial cells were gained after the administration of 0.2 % collagenase and the centrifugalization of the enzyme liquid. The cells were not cultivated in a 60 ml plastic culture until the presence of the second generation. The cultivated cells were confirmed as endothelial cells by factor 1/1[ related antigen. The multiplied cells were lined in vitro onto the luminal surface of allogenic vein that was disposed by freeze-drying and radiation. The orthotopic transplantation of autologous venous endothelial cells was performed after the 9-day incubation. Results （9.47±0.35） × 10^6 endothelial cells were obtained after the cultivation. Three hours after cell seeding, the luminal surface of allogenic vein was covered with vast endothelial cells but still had not formed an intact endomembrane. On day 9, the luminal surface was covered with a continuous endothelial monolayer and the arrangement and the shape of the cells all showed the perfect condition of endothelial cells. Eight weeks later, all the transplanted veins kept unobstructed. Conclusion The approach of lining allogenic vein with autologous endothelial cells in vitro may keep the vein unobstructed in the long term.

关 键 词：内皮细胞 异体血管 移植 

分 类 号：R654.3[医药卫生—外科学]