日本血吸虫成虫和虫卵可溶性抗原及其组分抗原的研究  

Analysis on soluble antigen and its fraction antigen of Schistosoma japonicum adult and egg

作　　者：姜旭淦[1] 傅行礼[1] 陈盛霞[1] 徐会娟[1] 帅连云[1] 曹建平[2] 仇锦波[1] 

机构地区：[1]江苏大学医学技术学院,江苏镇江212013 [2]中国疾病预防控制中心寄生虫病预防控制所,上海200025

出　　处：《江苏大学学报（医学版）》2007年第1期19-22,56,共5页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省高校自然科学基金资助项目(01KJB310012)

摘　　要：目的:探讨日本血吸虫雄虫、雌虫、虫卵的可溶性抗原雄虫(AWA-m、雌虫AWA-f、虫卵SEA)及其组分抗原的特性。方法:用DE22纤维素层析柱对日本血吸虫AWA-m,AWA-f,SEA抗原进行纯化,得到相应组分抗原。分别以SDS-PAGE和W estern b lot对组分抗原进行全面分析,并与相应未经纯化的可溶性抗原作比较。结果:经DE22纤维素层析处理的AWA-m,AWA-f,SEA,均能获得含多种蛋白的组分抗原。SDS-PAGE结果表明,AWA-m见10条主带和9条次带,其中Mr37 000,28 000,25 000为特异性条带,出现8条免疫反应阳性带;而AWA-m组分抗原为9条蛋白带和6条免疫反应阳性带。AWA-f见15条蛋白带,其中Mr26 500为特异性条带,组分抗原为8条蛋白带。AWA-f粗抗原及其组分抗原均见9条反应带。SEA见8条主带和10条次带,13条为免疫反应阳性条带;SEA组分抗原见11条带,其中9条为免疫反应阳性带。结论:AWA-m,AWA-f,SEA经DE22纤维素层析纯化获得组分抗原,方法简单、可靠。该组分抗原含有粗抗原的主要免疫反应成分,去除了多种与日本血吸虫病免疫反应无关的蛋白。Objective： To explore the characteristics of soluble male adult worm antigens （ AWA-m）, female adult worm antigens（ AWA-f）, egg antigens（SEA） and their fraction antigens of Schistosomia japonicum. Methods： The fraction antigens were isolated from AWA-m, AWA-f and SEA on DE,, cellulose column, analyzed by means of SDS-PAGE and western blot （ WB ）. Results： The three types of fraction antigens could be obtained from AWA-m, AWA-f and SEA on DE,, cellulose column. AWA-m and its fraction antigens were separated into 19 and 9 distinct bands in SDS-PAGE gel and revealed 8 and 6 immune bands on western blot respectively. AWA-f and its fraction antigens were separated into 15 and 8 distinct bands in SDS-PAGE gel and displayed 9 immune bands on WB. Most of AWA-m and AWA-f protein bands were similar, but Mr 37 000 and Mr 28 000 and Mr 25 000 bands were only appeared in AWA and Mr 26 500 band was seen in AWA-f. SEA and its fraction antigens were separated into 18 and 11 distinct bands respectively in SDS-PAGE gel and showed 13 and 9 immune reaction bands on WB. Condusion： The results suggest that our method for obtaining fraction antigens is simple and reliable by purifying AWA-m, AWA-f and SEA with DE22 cellulose. The fraction antigens contained the main immune components of crude soluble antigens and wiped out the major proteins irrespective with immune reaction of Schistosoma japonicum.

关 键 词：日本血吸虫 可溶性成虫抗原 可溶性虫卵抗原 组分抗原 

分 类 号：R383.2[医药卫生—医学寄生虫学]