新型螯合剂HBGD和BGD对铅致小鼠肝脏毒性的解毒作用研究  

Effects of new chelating agents HBGD and BGD on liver toxicity in mice induced by acute exposure to lead

作　　者：陈敏[1] 李伟剑[1] 宋霞[1] 张燕[1] 田晓雷[1] 谢吉民[1] 

机构地区：[1]江苏大学化学化工学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2007年第1期31-34,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省教育厅自然科学基金资助项目(00KJB330003)

摘　　要：目的:开发高效低毒的新型铅解毒剂。方法:观察铅染毒组小鼠和N-对羟甲苯甲基-D-葡糖二硫代氨基甲酸钠(HBGD)、N-苯甲基-D-葡糖二硫代氨基甲酸钠(BGD)、二乙基二硫代氨基甲酸钠(DDTC)、二巯丙醇(BAL)、乙二胺四乙酸(EDTA)等五种螯合剂治疗组小鼠血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)活性及小鼠肝脏脂质过氧化物(LPO)值和肝脏中铅浓度的变化。结果:醋酸铅染毒(40 mg/kg,尾静脉注射)后,小鼠血清LDH,AST,ALT活性及肝脏LPO值和铅浓度显著高于对照组。分别染铅30 m in和24 h后注射各螯合剂(400μmol/kg,腹腔注射),治疗24 h后HBGD和BGD对铅引起的血清LDH,AST,ALT活性及肝脏LPO值和铅浓度的升高有显著的抑制作用(P<0.05)。结论:HBGD和BGD对铅致小鼠肝脏毒性有较好的解毒作用,且自身毒性小,有望成为理想的铅解毒剂。Objective： To develop new antidotes of lead（Pb）. Methods： N-p-hydroxy-methyl-benzyl- D-glucamine dithiocarbamate （ HBGD ）, N-benzyl-D-glucamine dithiocarbamate （ BGD ）, diethyldithiocarbamate （ DDTC ）, 2,3-dimercaptopropanol （ BAL）, and ethylene diaminetetraacetic acid （EDTA） were studied for their effects on activity of lactate dehydrogenase （LDH）, aspartate transcarbamoylase （AST）, alanine aminotranferase （ALT） in serum , lipid peroxidation（LPO） and concentrations of Pb in liver of mice induced by acute exposure to lead. Results： Mice were injected intravenous with Pb （AC）2 （40 mg/kg） 30 min or 24 h later, then they were injected intravenous with chelating agents （400 μmol/kg）. Pb injection increased the activity of LDH, AST and ALT in serum and concentration of Pb in liver of mice. HBGD and BGD effectively prevented the increase of the activity of LDH, AST , ALT, LPO and concentrations of Pb （P 〈 0.05 ）. Conclusion： HBGD and BGD had some effective detoxification to liver toxicity in mice induced by acute exposure to Pb, So they may be good antidotes of Pb.

关 键 词：铅 螯合剂 肝脏 解毒作用 小鼠 

分 类 号：R595.2[医药卫生—内科学]