云南干热河谷地区余甘子居群的遗传多样性研究  

Genetic diversity of Phyllanthus emblica populations in dry-hot valleys in Yunnan

作　　者：李巧明[1] 赵建立[1] 

机构地区：[1]中国科学院西双版纳热带植物园植物系统与保护生物学实验室,昆明650223

出　　处：《生物多样性》2007年第1期84-91,共8页Biodiversity Science

基　　金：中科院方向性项目(KSCX2-SW-116);中科院西部之光项目和云南省基金(2002C0018Q)

摘　　要：余甘子(Phyllanthus emblica)是我国西南干热河谷地带的优势植物,也是一种重要的药用经济植物,对其遗传多样性进行研究对生态环境的建设和恢复有着重要意义。作者采用ISSR分子标记技术对分布于云南干热河谷地区的4个余甘子居群的遗传多样性水平进行了检测。12条引物共扩增出135条清晰、重复性好的DNA条带,其中多态性条带为115条,多态位点百分率(PPB)为85.19%。居群间的遗传分化系数(GST)为0.1222,基因流(Nm)为1.7958。结果表明余甘子居群具有较高的遗传多样性水平,而居群间存在较低的遗传分化,这可能主要是由其繁育特性造成的。Mantel检测表明地理距离和Nei's遗传距离间无相关性(r=0.19798,P=0.6513>0.05)。当我们进行干热河谷地区生态恢复时,以上结果对于确定余甘子的取样策略具有指导意义。Phyllanthus emblica （Euphorbiaceae） is one of the dominant species distributed in dry-hot valleys of southwestern China. Besides its function in ecology, environment and reforestation, it is also an important medicinal plant. We studied the genetic diversity in four populations of P. emblica sampled from dry-hot valleys of Yunnan using ISSR （inter-simple sequence repeats） markers. Based on 12 primers, 135 clear and reproducible DNA fragments were generated, of which 115 were polymorphic, accounting for 85.19%. The coefficient of genetic differentiation （GST） equaled 0.1222. Such a high level of genetic diversity and low level of population genetic differentiation might result from the breeding system of this species. There was a lack of significant association between genetic and geographical distances （r=0.19798, P=0.6513〉0.05） among P. emblica populations. The results suggest some important recommendations about sampling methods to be used in ecological reforestation of dry-hot river valleys.

关 键 词：药用经济植物 遗传结构 遗传分化 ISSR 

分 类 号：Q943[生物学—植物学]