6-巯基嘌呤-H_2O_2-Cu^(2+)-NaOH体系荧光分光光度法测定6-巯基嘌呤的研究  

Determination of 6-Mercaptopurine with 6-Mercaptopurine-H_2O_2-Cu^(2+)-NaOH System by Fluorimetry

作　　者：卢琴[1] 赵成安[1] 刘春[1] 徐洪伟[1] 王伦[1] 周运友[1] 

机构地区：[1]安徽省功能性分子固体重点实验室安徽师范大学化学与材料科学学院,安徽芜湖241000

出　　处：《分析科学学报》2007年第1期33-36,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.20575001);安徽省教育厅教学研究基金(No.JYXM2003174)

摘　　要：在碱性介质中,6-巯基嘌呤(6-MP)与过氧化氢反应可在405 nm波长处产生微弱的荧光,微量铜(Ⅱ)的存在能大大增敏其荧光强度。据此,建立了一种高灵敏测定6-MP的荧光光度分析新方法。在优化的条件下,体系的荧光强度与6-MP的浓度在2.1×10-8~1.7×10-5mol.L-1范围内呈线性关系,检出限为7.1×10-9mol.L-1。方法用于片剂中6-MP含量的测定,其结果与药典方法对照,令人满意。In basic media, 6-mercaptopurine reacts with hydrogen peroxide to form a new fluorescent compound,which possesses a weak emission wavelength of 405 nm. Its fluorescence intensity can be enhanced remarkably when trace Cu^2＋ exists as catalyst. Based on this, a novel spectrofluorimetric method with high sensitivity was developed for the determination of 6-mercaptopurine. Under the optimal conditions,the calibration graphs are linear over the range of 2.1 ×10^-8～1.7 ×10^-5 mol·L^-1 with a detection limit of 7.1×10^-9 mol·L^-1. The proposed method was successfully applied to determine 6-mercaptopurine in pharmaceutical preparations, the results obtained by pharmacopoeia method.

关 键 词：荧光光度法 6-巯基嘌呤 过氧化氢 铜(Ⅱ) 

分 类 号：O657.39[理学—分析化学]