高效液相色谱双波长法同时测定水产品中孔雀石绿和隐性孔雀石绿  

Determination of Malachite Green and Leucomalachite Green in Fishery Products by HPLC with Dual Wavelength Detector

作　　者：叶四化[1] 陆超华[1] 谢海平[1] 李娜[1] 

机构地区：[1]广东省海洋与渔业环境监测中心,广州510222

出　　处：《分析科学学报》2007年第1期69-72,共4页Journal of Analytical Science

摘　　要：通过紫外可见分光光度计扫描，发现孔雀石绿和隐性孔雀石绿的最大吸收波长分别为620nm和260nm。在相应的最大吸收波长处，采用高效液相色谱-双波长紫外可见检测器对水产品中孔雀石绿和隐性孔雀石绿同时测定。结果表明，水产品中孔雀石绿和隐性孔雀石绿回收率分别为83％～89％和87％～93％，相对标准偏差分别为2．12％～2．53％和2．16％～2．65％，检出限分别为1μg／kg和2μg／kg。Scaning by UV-Vis spectrophotometer, the maximum absorption wavelength of malachite green （λ：=620 nm） and leucomalachite green （22=260 nm） was found. Malachite green and leucomalachite green residues in fishery products were determined by HPLC at 2： and 22 with dual wavelength detector. The results showed that the recoveries of malachite green and leucomalachite green in fishery products were 83 % - 89% and 87% - 93%, the relative standard deviations were 2. 12% 2.53% and 2.16%-2.65% ,and the detection limits were 1 g/kg and 2 g/kg,respectively.

关 键 词：高效液相色谱 双波长 孔雀石绿 隐性孔雀石绿 

分 类 号：O657.72[理学—分析化学]