阿托伐他汀对THP-1巨噬细胞CD36表达的影响  

The effect of atorvastatin on CD36 expression in THP-1 macrophages

作　　者：曾颖[1] 谭玉林[1] 冯惊涛[1] 孙琳[1] 曾德星[1] 夏军辉[1] 王静[1] 尹小波[1] 易光辉[1] 

机构地区：[1]南华大学心血管疾病研究所,湖南省衡阳市421001

出　　处：《中国心血管病研究》2007年第2期132-134,共3页Chinese Journal of Cardiovascular Research

基　　金：国家自然科学基金资助项目(30570958);湖南省卫生厅课题基金资助项目(B2006-098)

摘　　要：目的研究阿托伐他汀(atorvastatin)对THP-1巨噬细胞B类清道夫受体CD36表达的影响。方法用5!mol/L阿托伐他汀与THP-1巨噬细胞孵育不同时间;用不同浓度的阿托伐他汀与THP-1巨噬细胞共同孵育24h,采用RT-PCR与Westernblotting分别检测THP-1巨噬细胞CD36mRNA与蛋白质的表达。结果阿托伐他汀使THP-1巨噬细胞CD36mRNA与蛋白质的表达下调(P<0.05),且时间与剂量呈依赖关系。结论阿托伐他汀能抑制THP-1巨噬细胞CD36的表达。Objective To investigate the effect of atorvastatin on CD36 expression in THP-1 macrophages. Methods THP-1 macrophages were treated with 5 μmol/L atorvastatin for 0 h,3 h,6 h,12 h,24 h respectively. And THP-1 macrophages were treated with atorvastatin at different concentrations （0 μmol/L,0.625 μmol/L, 1.2.5 p, mol/L,2.5 μmol/L,5 μmol/L）. CD36 mRNA and protein levels were determined by reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting respectively. Results We found that atorvastatin decreased CD36 in both protein and mRNA levels in a time-dependent and dose-dependent manner. Conclusion Atorvastatin may down-regulate CD36 expression in THP-1 macrophages.

关 键 词：阿托伐他汀 巨噬细胞 抗原 CD36 基因表达 动脉硬化 

分 类 号：R543.5[医药卫生—心血管疾病]