甲硫醇脱臭菌的分离、分子鉴定及应用  

Isolation,Application and Molecular Identification of Deodorizing Bacteria for Methyl Mercaptan

作　　者：刘波[1] 闫懂懂[1] 毕雪梅[2] 

机构地区：[1]南京大学污染控制与资源化国家重点实验室,江苏南京210093 [2]南京大学城市与资源学系,江苏南京210093

出　　处：《环境科学研究》2007年第1期104-107,共4页Research of Environmental Sciences

基　　金：国家自然科学基金资助项目(59978011);南京大学人才引进培养基金资助项目(0211005120)

摘　　要：以低浓度甲硫醇臭气的生物降解为研究对象,从不同的菌源中筛选得到一株能高效降解甲硫醇的菌株.运用16S rDNA的分子鉴定技术,确定筛选菌Jll的16S rDNA序列同芸苔根际菌(Rape rhizosphere)的相似性最大(97%),为同属.筛选菌Jll生长迅速,挂膜时间短.将其接种于生物滴滤反应器中,在甲硫醇进气流量为0.3 m3/h,质量浓度为40 mg/m3,运行仅4 d后,其去除率可逐渐升到100%.当进气流量分别为0.3,0.6和1.2 m3/h时,该反应器对甲硫醇的最大去除能力分别为36.2,41.8和16.4mg/(m3.h).反应器进气负荷提高到(96±3)mg/(m3.h),运行一段时间后,恢复适宜的进气负荷,生物滴滤反应器对甲硫醇的去除率很快回升.这表明菌Jll不但降解能力高,而且抗逆性强.The objective of this research is to degrade low concentration Methyl mercaptan by microorganism. Strain Jll screened out from different odor origins could efficiently remove methyl mercaptan （MM）. A systematic identification method, 16S rDNA gene sequence comparison, for deodorizing bacteria was carried .out. The 16S rDNA gene sequence analysis of strain JU showed the highest level of 97% homology to Rape rhizosphere. The strain Jll＇ s growth was rapid and the time of deodorizing domestication shortened. After JU was inoculated into trickled-bed biofilters, the removal efficiency was up to 100% after only 4 days, operating at the air flow rate of 0.3 m^3/h and inlet pollutant concentration of 40 mg/m^3 . When the superficial air flow rate was 0.3, 0.6 and 1.2 m^3/h, maximum elimination capacity of 36.2, 41.8 and 16.4 mg/（m^3 ·h） was obtained respectively. Adjusting inlet loads of （96 ± 3） mg/（m^3·h） to suitable low inlet loads, the removal efficiency of bioreactor could improve rapidly. It shows that the Jll has powerful capacity to degrade MM and resistance to shocks.

关 键 词：16S RDNA基因 甲硫醇 芸苔根际菌(Rape rhizosphere) 生物滴滤反应器 

分 类 号：X512[环境科学与工程—环境工程]