维拉帕米和生脉注射液抗大鼠肝脏缺血/再灌注损伤的作用研究  

Study of protective effect of verapamil and Shengmai injection (生脉注射液) on ischemia/reperfusion injury in rat liver

作　　者：山峰[1] 李堃[1] 马承泰[1] 韩小宁[1] 孙运波[1] 吴力群[1] 王静[2] 王秋波[2] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003 [2]青岛大学医学院分子与细胞免疫学教研室,山东青岛266003

出　　处：《中国中西医结合急救杂志》2007年第1期35-38,F0002,共5页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：山东省卫生厅青年基金资助项目(2001CAZCKA1)

摘　　要：目的:观察维拉帕米和生脉注射液对大鼠肝脏缺血/再灌注(I/R)损伤的保护作用。方法:将40只大鼠随机分为假手术组、I/R组、维拉帕米组和生脉注射液组,每组10只。采用夹闭左肝蒂30min后开放再关腹制备大鼠肝I/R损伤模型。维拉帕米组开腹前10min经尾静脉注射维拉帕米1.25mg/kg;生脉注射液组开腹前3d每日向腹腔内注射生脉注射液5ml/kg。各组均在关腹48h后再开腹,每只大鼠取缺血肝组织并由腹主动脉抽血,检测丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、热休克蛋白70(HSP 70)的变化;并在光镜下观察肝组织的形态变化。结果:与假手术组比较,I/R组ALT、AST、TNF-α和MDA水平均显著升高(P均<0.05);维拉帕米组和生脉注射液组ALT、AST、TNF-α和MDA均显著低于I/R组,但显著高于假手术组(P均<0.05);而维拉帕米组和生脉注射液两组之间除ALT有差异外,其余指标差异无显著性。维拉帕米组和生脉注射液组HSP 70的表达均明显少于I/R组,而维拉帕米组HSP 70的表达略少于生脉注射液组。肝组织切片观察显示,维拉帕米组和生脉注射液组肝细胞肿胀、坏死程度均较I/R组轻。结论:维拉帕米和生脉注射液可明显改善大鼠肝脏I/R后肝组织的损伤程度。Objective： To observe the protective effect of verapamil and Shengmai injection （生脉注射液） on ischemia/reperfusion （I/R） injury in rat liver. Methods： A rat model of liver I/R injury was established. Ischemia of the left lobe was created by total inflow occlusion for 30 minutes followed by perfusion. Forty healthy Wistar rats were randomly divided into four groups as follows： sham operation group, I/R group, verapamil group pretreated with verapamil （1.25 mg/kg） by caudal vein injection 10 minutes before ischemia and Shengmai injection group pretreated with Shengmai injection （5 ml · kg^-1· day^-1） by intraperitoneal injection for 3 days before ischemia. Forty-eight hours after I/R, the liver tissues were obtained for light microscopic study and blood was drawn for examinations. The heat shock protein 70 （HSP70） expression level was determined by Western blotting. Malondialdehyde （MDA） was measured by TBA colorimetric method. Serum levels of tumor necrosis factor - α （TNF - α） was determined using radioimmunoassay kit, and alanine aminotrasferase （ALT） and aspartate aminotrasferase （AST） were measured by full-automatic biochemical analyzer. Results： Compared with sham operation group, levels of ALT, AST, TNF -α and MDA were significantly increased in I/R group （all P〈0. 05）. The levels of ALT, AST, TNF -α and MDA in verapamil group and in Shengmai injection group were significantly lower than those in I/R group and significantly higher than those in sham operation group （all P〈0.05） , but no significant differences were found between verapamil group and Shengmai injection group except for ALT. HSP70 expression levels in verapamil group and Shengmai injection group were obviously lower than that in I/R group, and slightly lower in verapamil group than that in Shengmai injection group. The liver cellular edema, necrosis degree in verapamil group and Shengmai injection group were effectively alleviated compared with those in I/R group. Conclusion�

关 键 词：维拉帕米 生脉注射液 缺血/再灌注损伤 肝 

分 类 号：R96[医药卫生—药理学] R285.5[医药卫生—药学]