两个亚洲百合品种离体再生体系的建立  

Establishment of regeneration system on two species of Lilium asiatic hybrid in vitro.

作　　者：周艳萍[1] 郑红娟[1] 贾桂霞[1] 

机构地区：[1]北京林业大学林木花卉遗传育种教育部重点实验室

出　　处：《北京林业大学学报》2007年第1期123-127,共5页Journal of Beijing Forestry University

基　　金：国家转基因植物研究与产业化开发专项(JY04--B--02);高等学校博士学科点专项科研基金资助课题(20050022015)

摘　　要：以亚洲百合‘普瑞头’、‘布耐罗’2个品种的外中层鳞片为外植体，培养基以MS为基础附加不同浓度的6-BA及NAA，成功诱导出了不定芽，得出：‘普瑞头’鳞片再生较适宜的生长调节剂配比为MS＋6-BA2．0mg／L＋NAA0．2mg／L（单位下同），‘布耐罗’为MS＋6-BA0．5＋NAA1．0．通过对再生苗叶片不同部位的再分化实验，比较得出小鳞茎叶的叶片基部分化能力非常强，而叶片上部基本不分化，鳞茎叶基部是建立高频快速再生体系的首选材料．该文进一步探讨了适宜鳞茎叶基再生的生长调节剂配比，适宜两个品种鳞茎叶基再生的生长调节剂配比为：MS＋6-BA2．0＋NAA0．2、MS＋6-BA1．0＋NAA1．0、MS＋6-BA0．5＋NAA0．5．最后，研究了母株不同继代次数对叶基不定芽再生率的影响，结果表明：母株继代培养5—6代后，叶基的分化能力略微下降，约为80％．Using the outer and middle layers of bulb scales as the explants, plant regeneration system of two Lilium asiatic hybrid--‘ Prato＇ and ‘Brunello＇ , was established from culturing on Murashige and Skoog＇s basal medium supplemented with 6-BA and NA_A at different concentrations, and the optimum media for shoot proliferation were： MS medium supplemented with 6-BA 2.0 mg/L and NAA 0.2 mg/L for ‘Prato＇ , MS with 6-BA 0.5 rng/L and NAA 1.0 mg/L for ‘Brunello＇. The differentiation ability for different parts of bulblet leaves was studied, and the results showed that the apical part of leaves did not regenerate, however, mass shoot regeneration occurred from the leaf base, occasionally from the middle part. MS supplemented with 6- BA, NAA and 2, 4-D at different concentrations was carried out to select a suitable method for shoot regeneration. As a result, all the media induced shoot regeneration via direct organoensis, but the medium with 2,4-D manifested no positive effects. Three kinds of suitable media, using base of bulblet leaves for proliferation were： MS medium supplemented with 6-BA 2.0 mg/L and NAA 0.2 mg/L, or with 6-BA 1.0 mg/L and NAA 1.0 mg/L, or with 6-BA 0.5 mg/L and NAA 0.5 mg/L. The leaf bases which have been separated from the sueeeeding-euhured mother plantlet for 5-6 times could still have a high percentage about 80% of shoot regeneration.

关 键 词：亚洲百合 组培 再生体系 鳞片 小鳞茎叶 

分 类 号：S682.2[农业科学—观赏园艺]