红霉素对体外培养人鼻息肉上皮细胞凋亡的影响  

Erythromycin effects on apoptosis of epithelial cell in nasal polyps

作　　者：杨雷[1] 徐赛男[2] 金永国[1] 

机构地区：[1]温州医学院生物学技术平台,浙江温州325000 [2]温州市第三人民医院五官科

出　　处：《临床耳鼻咽喉头颈外科杂志》2007年第2期84-85,共2页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:观察红霉素对上皮细胞凋亡的影响,进一步探讨上皮细胞凋亡在鼻息肉组织形成中的意义。方法:将30例鼻息肉及6例下鼻甲的上皮细胞均分成2组,其中1组加红霉素(红霉素组),另一组不加红霉素(对照组),进行原代上皮细胞的培养,1、3、5d后应用TUNUL法检测细胞凋亡。结果:红霉素组培养1、3、5d的上皮细胞凋亡指数分别为(33.23±6.50)%、(38.21±7.22)%和(52.63±7.86)%,对照组为(31.02±5.60)%、(32.13±7.15)%和(39.64±7.48)%,培养5d的上皮细胞凋亡指数差异有统计学意义(P<0.05)。结论:红霉素有显著的促鼻息肉上皮细胞凋亡的作用。Objective：To study the effect of erythromycin on apoptosis in epithelial cell and investigate the significance of epithelial cell apoptosis in nasal polyps forming. Method： Epithelial cell collected from thirty nasal polyps and six inferior turbinates were cultured in Dulbecco Eagle and Ham F12（1 ： 1） and divided into two groups, one culured with Erythromycin（Erythromycin group）, another cultured without Erythromycin（control group）. Ap- optosis was detcted by terminal deoxynucleotidyl transferasemediated dUTP nick endlabelinn. Result： The AI （apoptosis index） of epithelial cell in nasal polyps after cultured for 1,3,5 days with erythromycin were respectively （33.23±6.50）% ,（38.21±7.22）% and （52.63±7.86）%. The AI of epithelial cell in inferior turbinates were respectively （31. 025b 5.60） %, （32. 13 ± 7.15） % and（39.64 ± 7.48） %. There were significant difference between two groups at 5 day after culture（ P 〈0.05）. Conclusion：Erythromycin promoted apoptosis of epithelial cell in nasal polyps.

关 键 词：红霉素 鼻息肉 上皮细胞 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]