阻断协同刺激分子对MMP-9/TIMP-3的表达及妊娠结局的影响  

Effect of Blockage of Costimulatory Signal CD86 on the Expressions of MMP-9/TIMP-3 Proteins and Outcome of Pregnancy

作　　者：赵富玺[1] 张源源[1] 刘润花[2] 李拴明[1] 崔克[1] 刘丽华[1] 

机构地区：[1]山西大同大学医学院病原生物与免疫学研究所 [2]山西大同大学医学院外科研究所,大同037008

出　　处：《生殖与避孕》2007年第1期26-30,共5页Reproduction and Contraception

基　　金：国家人口科[2005]11号(C1-41)

摘　　要：目的：探讨阻断CD86协同刺激分子对自然流产模型孕鼠母胎界面MMP-9和TIMP-3的表达及妊娠结局的影响。方法：实验组于妊娠d 4．5腹腔注射大鼠抗小鼠CD86单抗,对照组注射大鼠同型IgG2b,而正常组不作任何处理。于妊娠d 13．5计算胚胎吸收率,并用免疫组化测定MMP- 9和TIMP-3的表达。结果：实验组的胚胎吸收率和MMP-9均显著低于对照组(P＜0．05),与正常对照组间均无差异；TIMP-3的表达与对照组和正常组比较均无显著差异(P＞0．05)。结论：在妊娠早期阻断CD86协同刺激分子能够通过某些机制诱导自然流产鼠MMP-9/TIMP-3的比值降低并且降低自然流产模型的胚胎吸收率,使模型组的胚胎吸收率恢复至正常妊娠水平。Objective： To investigate the effect of blockage of costimulatory signal CD86 at the time of implantation on the expressions of MMP-9 and TIMP-3 proteins at the maternal-fetal interface and outcome of pregnancy in murine abortion-prone model. Methods： Female pregnant CBA/J mice in experimental group received an intraperitoneal （i.p） injection of 100 μg of anti-mouse CD86 mAb in 200 μl of PBS at d 4.5 of gestation, and the irrelevant-isotope matched rat IgG2b was administrated in control group with the same dosage and at same time. For normal group, no treatment was given. The pregnant CBA/J mice were killed on d 13.5 of gestation.Then, the embryo resorption rate was calculated and the expressions of MMP-9 and TIMP-3 proteins were detected by using imrnunohistochemical methods. Results： The embryo resorption rate and the expression of MMP-9 in experimental group significantly were reduced compared with those in control group（P〈0.05）and had no significantly difference from those in normal group（P〉0.05）. The expression of TIMP-3 in experimental group had no significantly difference from that in control group and normal group （P〉0.05）.Conclusion： Blockage of costimulatory signal CD86 at the early stage of gestation can induce the reduction of the ratio of MMP-9/TIMP-3 by some mechanisms and reduce the embryo resorption rate in the abortion-prone model to that of normal pregnant level.

关 键 词：免疫 耐受 胚胎 妊娠 流产 

分 类 号：R714.1[医药卫生—妇产科学]