花粉管通道法导入标记DNA在棉花胚珠内的分布  

DISTRIBUTION OF EXOGENOUS DNA IN UPLAND COTTON BY POLLEN TUBE PATHWAY

作　　者：杨书华[1] 倪万潮[1] 葛才林[2] 朱静[2] 王泽港[2] 罗时石[2] 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014 [2]扬州大学农学院,江苏扬州225009

出　　处：《核农学报》2007年第1期13-16,共4页Journal of Nuclear Agricultural Sciences

基　　金：农业部发展棉花生产专项基金(005032910102)

摘　　要：通过花粉管通道法将[α3-2P]dATP标记的DNA导入科棉1号,测量标记DNA在胚珠内的放射性活度并进行放射性自显影观察,以求获得陆地棉导入外源DNA的最佳时期和外源DNA在胚珠内的分布情况。结果表明,导入的外源DNA能进入到胚珠中;棉花授粉33h时通过滴涂和注射法导入标记DNA在胚珠内的放射性活度测量值均较高;而滴涂法的放射性活度明显低于注射法;胚珠内银粒的分布较明显,标记外源DNA的胚珠内银粒远多于对照。The distribution of exogenous DNA in upland cotton by pollen tube pathway was studied. The results showed that the exogenous DNA could be transported into ovules by dripping and injection. The optimum time for treatment is at 33h after pollination.The radioactivity in ovules by dripping exogenous DNA was lower than that by injecting. In autoradiogram, the number of sliver grains in the treated ovules was more than that in the controls.

关 键 词：陆地棉 花粉管通道 放射性自显形法 

分 类 号：S562[农业科学—作物学]