一株极端耐辐射奇异球菌的分离和初步鉴定  

SEPARATION AND CHARACTERIZATION OF A RADIORESISTANT BACTERIUM STRAIN BR501 FROM RADIATION POLLUTED SOIL

作　　者：陈明[1] 刘秀敏[1] 张维[1] 林敏[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《核农学报》2007年第1期44-47,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家高技术研究发展计划项目(2004AA214170)

摘　　要：从放射性污染的土壤样品中分离出1株具有极端耐辐射的微生物BR501,该菌最适生长温度为30℃,不具有氨苄青霉素、氯霉素、四环素、卡那霉素和利福平等抗性。UV、γ辐射生长曲线的结果显示BR501菌株具有极强的辐射抗性。BR501菌株的16S rDNA序列与多株Deinococcus属菌株有很高的同源性,其中,与D.radiodurans菌株的16S rDNA相似性高达99%。结合BR501菌株的表型和Deinococcus属具有代表性的D.RadioduransR1菌株的特征,将该菌株归属为Deinococcus属,初步命名为Deinococcus sp.BR501。Strain BR501, an extremely radioresistant bacterium isolated from the radioactive experimental soil. The optimal temperature for the growth of strain BR501 was 30℃. The UV radiation and T-radiation survival curves showed the strain BR501 had highly radio-resistance. The strain was sensitive to Amp, Km, Rif, Cm and Tc. The 16S rDNA of the BR501 shared highly similarity to those of species in genus Deinococcus, especially to that of D. radiodurans R1 （99%）. Based on the 16S rDNA sequence analysis and the phenotype characteristics, the BR501 belongs to the evolution branch of Deinococcus and was designated Deinococcus sp. BR501.

关 键 词：耐辐射微生物 DEINOCOCCUS SP 16S RDNA 

分 类 号：Q93-33[生物学—微生物学]