L-α-二油酸磷酯酰胆碱和芦丁硬脂酸酯混合单分子膜的相容性  

Miscibility of DOPC/RS monolayers at the air/water

作　　者：段煜[1] 杜宗良[1] 唐卫文 李瑞霞[1] 吴大诚[1] 

机构地区：[1]四川大学轻纺与食品学院,四川成都610065 [2]成都地奥制药集团有限公司,四川成都610000

出　　处：《化学研究与应用》2007年第2期183-187,共5页Chemical Research and Application

摘　　要：Rutin was acylated with stearic acid in esterification reaction catalyzed by immobilized Candida antarctica lipase B(Novozym 435).The results suggested that the regioselectivity of the lipase-catalyzed esterification of rutin was specific at the C4’’’ position of the rhamnose moiety.The monolayer of dioleoylphosphatidylcholine(DOPC) was used as the model membrane to study their molecular interaction with RS.The miscibility of DOPC/RS mixed monolayers has been investigated by Langmuir film balance to yield quantitative information on the nature of the molecular interaction,which could provide valuable insights to reveal the antioxidative activity mechanism of flavonoids.Pure RS and pure DOPC were capable of forming monolayers with liquid-phase at the air/water interface.The two components of DOPC/RS monolayer were miscible throunghout the mixture composition range.The effects of the temperature of the subphase on the miscibility and molecular interaction of DOPC/RS monolayers were investigated.The miscibility was more excellent at higher temperature.The condensing effect of RS,which represented the attractive forces between DOPC and RS,could be ascribed to the match of molecular shapes of RS and DOPC on the monolayers packing.Rutin was acylated with stearic acid in esterification reaction catalyzed by immobilized Candida antarctica lipase B （ No- vozym 435 ）. The results suggested that the regioselectivity of the lipase - catalyzed esterification of rutin was specific at the C4- position of the rhamnose moiety. The monolayer of diolcoylphosphatidylcholine （DOPC） was used as the model membrane to study their molecular interaction with RS. The miscibility of DOPC/RS mixed monolayers has been investigated by Langmuir film balance to yield quantitative information on the nature of the molecular interaction, which could provide valuable insights to reveal the antioxidative activity mechanism of flavonoids. Pure RS and pure DOPC were capable of forming monolayers with liquid - phase at the air/water interface. The two components of DOPC/RS monolayer were miscible throunghout the mixture composition range. The effects of the temperature of the subphase on the miscibility and molecular interaction of DOPC/RS monolayers were investigated. The miscibihty was more excellent at higher temperature. The condensing effect of RS, which represented the attractive forces between DOPC and RS,could be ascribed to the match of molecular shapes of RS and DOPC on the monolayers packing.

关 键 词：混合单分子膜 芦丁硬脂酸酯 L-α-二油酸磷脂酰胆碱 表面压-分子面积等温线 相容性 分子间相互作用 

分 类 号：O647.11[理学—物理化学]