游离脂肪酸对肾小管上皮细胞TIMP-1、PAI-1表达及细胞外基质分泌的影响  

The effects of free fatty acids on the gene expression of extracellular matrix degradation enzyme inhibitors and the secretion of extracellular matrix in renal tubular epithelial cells

作　　者：黄志文[1] 梁东[1] 刘华锋[1] 唐德燊[1] 刘海燕[1] 

机构地区：[1]广东医学院附属医院肾病研究所,广东湛江524001

出　　处：《中国实验诊断学》2007年第2期177-180,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨游离脂肪酸(Free fatty acids,FFAs)对人肾小管上皮细胞(renal tubular epithelial cells,RTECs)细胞外基质降解酶抑制剂〔tissue inhibitor of metalloproteinase 3/1(TIMP-1)、plasminogen activator inhibitor 3/1(PAI-1)〕表达及细胞外基质〔纤维连接蛋(fibronectin,FN)、胶原〕分泌的影响。方法用去脂牛血清白蛋白(defatty bovine serum albumin,d-BSA)为油酸(Oil acids,OA)载体,用不同浓度OA刺激培养RTECs,以不加d-BSA及只加普通培养液的细胞作对照组,应用反转录多聚酶链反应(RT-PCR)检测RTECs TIMP-1 mRNA、PAI-1 mRNA的表达水平,酶联免疫吸附法(ELISA法)、羟脯氨酸消化法分别检测培养上清FN、胶原的蛋白水平。结果d-BSA组与空白对照组RTECs的细胞外基质降解酶抑制剂(TIMP-1 mRNA、PAI-1 mRNA)表达及细胞外基质蛋白(FN、胶原)分泌比较差异无显著性(P>0.05),而不同浓度OA组与空白对照组比较差异有显著性(P<0.05);且随着OA浓度增加,对RTECs TIMP-1 mRNA、PAI-1 mRNA表达以及对FN、胶原蛋白分泌的促进作用有增强趋势。结论FFAs通过促进细胞外基质(FN、胶原)的生成及抑制其降解,使其沉积在肾间质,进而在肾小管间质纤维化发生发展中起着重要的作用。Objective To study the effects of free fatty acids （FFAs） on gene expression of extracellular matrix degradation enzyme inhibitors （ tissue inhibitor of metalloprotcinase- 1 （TIMP- 1 ）, and plasminogen activator inhibitor- 1 （PAI- 1 ） ） and the secretion of cxtracellular matrix （fibronectin（FN） ,collagen） protein in proximal renal tubular epithelial cells （RTECs）.Methods OA were carfled by defatty bovine serum albumin（d-BSA）.Cells were exposed to normal medium and different concentrations of OA which using d- BSA as vector. The gene expression of TIMP-1 ,PAI-1 were detected by reverse transcripase polymerase chain reaction （IRT-PCR） , the excretion of FN and collagen protein were assessed by ezyme link immunosordent assay （FLISA） and Hydroxyproline digestion method respectlvely.Results There was no differences in the gene expression of TIMP-1,PAI-1 and the excretion of FN and collagen protein between cells exposed to normal medium and medium just with OA vector .OA can significantly up-regulated the gene expression of TIMP- 1 and PAI- 1 as well as the secretion of FN and collagen protein（ P 〈 0.05）. Conclusion FFAs may promote ECM deposition via promoting ECM production and inhibiting ECM catabolism by RTECs.

关 键 词：油酸 肾小管上皮细胞 细胞外基质降解酶抑制剂 纤维连接蛋白 胶原 

分 类 号：R692.6[医药卫生—泌尿科学]