醋酸铅对PC-12细胞Bax、Bcl-2和p53蛋白表达的影响  

Influence of lead acetate on the expressions of Bax, Bcl-2 and p53 in PC-12 cells

作　　者：徐进[1] 季林丹[1] 徐立红[1] 

机构地区：[1]浙江大学医学院生物化学与遗传学系,杭州310058

出　　处：《环境科学学报》2007年第2期272-275,共4页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金重点项目(No.20137010)~~

摘　　要：用蛋白免疫印迹法研究醋酸铅对PC-12细胞Bax、Bcl-2和p53蛋白表达的影响.结果显示,当醋酸铅浓度达到0.1μmol·L-1时,Bax和p53的表达水平显著上升,决定凋亡是否发生的重要因素Bax/Bcl-2比率也明显上升,并存在浓度依赖关系.当醋酸铅浓度达到1μmo·lL-1时,Bcl-2的表达显著降低.结合相关文献报道,Bax、Bcl-2和p53可能在铅诱导的细胞凋亡中起了重要的调控作用.The expressions of Bax, Bcl-2, and p53 in PC-12 cells after exposure to lead acetate were detected by Western blot. The results showed that, when treated with 0.1 μmol·L^-1 lead acetate, the expressions of Bax and p53 increased significantly. Furthermore, the ratio of Bax/Bcl-2, an important determinant of apoptosis, increased in a concentration-dependent manner. When the concentration of lead acetate reached 1 mol·L^-1 , the expression of Bcl-2 decreased significantly. Together with our previous study, it suggests that Bax, Bcl-2, and p53 may play important roles in lead-induced apoptosis.

关 键 词：醋酸铅 PC-12细胞 BAX BCL-2 P53 

分 类 号：R994.6[医药卫生—毒理学]