家兔阴茎皮肤和阴茎背神经躯体感觉神经元的示踪定位  

Location of Somatic Sensory Neurons of the Skin and Dorsal Nerve of the Penis in Rabbits

作　　者：吴包金[1] 江华[1] 李文鹏[1] 张盈帆[1] 陈刚[1] 

机构地区：[1]第二军医大学附属长征医院整形外科,上海200003

出　　处：《中华男科学杂志》2007年第1期17-20,共4页National Journal of Andrology

基　　金：上海市科技发展基金(024119057)

摘　　要：目的：明确家兔阴茎感觉神经躯体纤维来源及其节段性分布规律。方法：健康成年雄性新西兰家兔8只，随机均分为A、B两组，采用辣根过氧化物酶（HRP）逆行神经示踪技术，A组用微量注射器将50％HRP多点注射于阴茎皮下，B组注射于右侧阴茎背神经近侧断端内，留针时间30min，动物存活5d后，4％多聚甲醛心脏灌注，取出腰骶段脊髓及相应背根神经节（DRG），对躯体传入和传出纤维来源的节段分布进行观察。结果：A组s2一s。节段脊神经节内出现大量HRP阳性标记感觉神经元，以S：和S。为多，呈明显节段性分布，各节段阳性神经元计数分别为：S：（215．0±10．2）、S，（242．2．4-8．3）、S。（109．7．4-8．4），组间差异具有统计学意义（P〈0．01）。B组同侧S2-S4节段脊髓灰质前角腹外侧部出现部分阳性标记细胞，同侧S2-S4 DRG内也出现了大量的HRP阳性细胞。结论：家兔阴茎皮肤感觉神经躯体传入和传出纤维投射来源与阴茎背神经一致，均源自骶髓前角及相应的脊神经节，并呈现明显的节段性分布。Objective： To trace the segmental distribution of somatic sensory neurons of the skin and dorsal nerve in the rabbit＇s penis. Methods ： The experiment was performed on 8 adult male rabbits with the nerve tracing method of retrograde axonal transport of horseradish peroxidase （ HRP）, which was injected into the dermis around the penis and the dorsal nerve of the penis. The rabbits were sacrificed five days later to havest the spinal cord segments and the dorsal root ganglia of lumbosacral segments for histological study. Resuits ： The HRP tracing showed that a number of labeled HRP positive neurons appeared in spinal ganglia （ S2 - S4 ） in all the rabbits, and distributed segmentally. The counts of the positive neurons different segments were： S2 （215.0 ± 10.2） , S3 （ 242.2 ± 8.3 ） and S4 （109.7 ± 8.4） respectively, with statistically significant difference between the two groups. Conclusion： The rabbits sensory nerve fibers in beth the skin and the dorsal nerve of the penis are rooted in the S2-S4 segments of spinal ganglia, which distribute regularly.

关 键 词：阴茎 感觉神经 阴茎背神经 辣根过氧化物酶 兔 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]