不育患者血清精子蛋白17抗体的检测及临床意义  

Detection of Anti-Sp17 Antibodies in Infertile Patients' Serum and Its Clinical Significance

作　　者：张春华[1] 李芳秋[1] 杨爱龙[1] 孙伟[1] 缪家文[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所

出　　处：《中华男科学杂志》2007年第1期27-29,共3页National Journal of Andrology

基　　金：江苏省医学重点学科基金资助项目(苏卫科教[2001]34)

摘　　要：目的:检测抗精子抗体(AsAb)阳性不育患者血清中抗精子蛋白17(Sp17)的抗体,探讨该抗体在免疫性不育血清学诊断和免疫避孕方面的潜在应用价值。方法:用重组人Sp17作为抗原,ELISA法检测62例AsAb阳性血清中Sp17抗体阳性率及滴度,分析AsAb中抗Sp17的含量。结果:AsAb阳性血清中Sp17抗体阳性率为56.5%,男女之间差异无显著性。Sp17抗体占AsAb的(10.09±7.45)%,结果具有统计学意义(P<0.05)。结论:Sp17是重要的精子抗原组分,血清中Sp17抗体的检测可作为不育患者辅助诊断指标,同时也提示Sp17可能是一种免疫避孕候选抗原疫苗。Objective： To test anti-Sp17 antibodies in the serum of AsAb positive infertile patients, to investigate the proportion of anti-Sp17 antibodies in AsAb and their potential application to the serologic diagnosis of immune infertility and immunocontraception. Methods： With human recombinant Sp17 as the antigen, the ELISA method was used to detect the positive rate, antibody titre and content of anti-SpiT antibodies in the AsAb positive serum. Results： The positive rate of anti-SpiT antibodies in the AsAb positive serum was 56.5%, with no significant difference in the gender aspect. The percentage of anti-SpiT antibodies in AsAb was （ 10.09 ± 7.45） %, with statistical significance （P 〈 0.05）. Conclusion： Sp17 is an important sperm antigen. Anti-Sp17 antibodies in the serum can be taken as auxiliary diagnostic index of infertility, and Sp17 is shown to be a potential candidate immunocontraception vaccine.

关 键 词：精子蛋白17 抗体 免疫性不育 免疫避孕 

分 类 号：R698.2[医药卫生—泌尿科学]